X-Ray Methods in Structural Biology
October 15 - 30, 2019
Application Deadline: June 15, 2019
Janet Newman, CSIRO, Australia
James Pflugrath, Rigaku Americas Corporation (ret.), Texas
Anastassis Perrakis, Netherlands Cancer Institute
Paul Adams, Lawrence Berkeley National Laboratory
X-ray crystallography has been the cornerstone of structural biology for half a century, and remains the technique of choice for atomic resolution understanding of macromolecules and for structure guided drug discovery. This intense course combines laboratory and computational instruction to train course participants in the major techniques used to determine three-dimensional structures. It is designed for scientists with a working knowledge of protein structure and function, but who are new to macromolecular crystallography or who wish to increase their in-depth knowledge of macromolecular crystallography.
Topics to be covered include:
- Basic diffraction theory
- Crystallization (proteins, nucleic acids, complexes and membrane proteins)
- Synchrotron X-ray sources and optics
- Data collection and processing
- Structure solution by experimental phasing methods (SAD, MAD, MIR, and others) and molecular replacement
- Electron density maps improvement
- Model building and refinement
- Structure validation
- Coordinate deposition
- Structure presentation
Participants have extensive hands-on training in well-equipped labs in how to crystallize multiple proteins and determine their crystal structures by several methods, while learning through lectures on theory and methods. Both basic and advanced subjects are covered during lectures, which are given by leaders in the field. Informal discussions behind the techniques are frequent and students are expected to pose questions to be answered in interactive sessions.
Applicants should be familiar with the creation and editing of simple text files on Linux workstations.
2018 Speakers Included:
Dominika Borek, UT Southwestern Medical Center at Dallas
Martin Caffrey, Trinity College Dublin, Ireland
Bil Clemons, California Institute of Technology
James Conway, University of Pittsburgh
Paul Emsley, MRC Laboratory of Molecular Biology, United Kingdom
Gary Gilliland, Janssen Research & Development, LLC
Wayne Hendrickson, Columbia University
James Holton, University of California, San Francisco
Gerard Kleywegt European Bioinformatics Institute, United Kingdom
Janet Newman, CSIRO, Australia
Randy Read, University of Cambridge, United Kingdom
Jane Richardson, Duke University
Clyde Smith, Stanford University
Robert Sweet, Brookhaven National Laboratory
Thomas Terwilliger, Los Alamos National Laboratory
Andrea Thorn, University of Hamburg, Germany
Dale Tronrud, The Light and Electron Works
Christopher Williams, Duke University
This course is supported with funds provided by: National Institute of General Medical Sciences.
Support & Stipends:
Stipends are available to offset tuition costs as follows-
Please indicate your eligibility for funding in your stipend request submitted when you apply to the course. Stipend requests do not affect selection decisions made by the instructors.
Cost (including board and lodging): $4,345
No fees are due until you have completed the full application process and are accepted into the course. Students accepted into the course should plan to arrive by early evening on October 14 and plan to depart after lunch on October 30.
Before applying, ensure you have:
- Personal statement/essay;
- Letter(s) of recommendation;
- Curriculum vitae/resume (optional);
- Financial aid request (optional).
If you are not ready to fully apply but wish to express interest in applying, receive a reminder two weeks prior to the deadline, and tell us about your financial aid requirements, click below: