Advances in light microscopy, digital image processing, and the development of a variety of powerful fluorescent probes present expanding opportunities for investigating the nervous system, from synaptic spines to networks in the brain. This intensive laboratory and lecture course will provide participants with the theoretical and practical knowledge to utilize emerging imaging technologies. The primary focus of the course will be on in vivo applications of light microscopy, particularly functional imaging with genetically encoded calcium indicators. Methods taught will include multi-photon and light-sheet microscopy, and combination of imaging with optogenetics. Lectures by leading experts will progress through basic concepts to presentation of cutting edge methods. Students will learn the fundamentals of optics and microscopy, as well as the use of different types of cameras, laser scanning systems, in vivo preparations and image processing and analysis software. A strong emphasis is placed on building exercises that allow students to develop an understanding of basic principles, while also introducing them to a variety of state-of-the-art commercial systems. 

Applicants should have a strong background in the neurosciences or in cell biology. In their personal statements, applicants should specify: 1) their experience with optical techniques, 2) how they will apply optical methods in their current projects, 3) the microscope systems available to them, and 4) their long-term goals in learning more about optical methods.

Major support provided by the Helmsley Charitable Trust and Howard Hughes Medical Institute

Stipends are available to offset tuition costs as follows:

Interdisciplinary Fellowships (transitioning from outside biology)  & Scholarships (transitioning from other biological disciplines) (Helmsley Charitable Trust)
Domestic/International applicants (Howard Hughes Medical Institute)

Scientists from developing countries accepted into this course may be eligible for scholarships provided by the International Brain Research Organization.

Please indicate your eligibility for funding in your stipend request submitted when you apply to the course. Stipend requests do not affect selection decisions made by the  instructors.