Meetings & Courses Program

Crossroads of Biology

Mouse Development, Stem Cells & Cancer
June 8 - 27, 2022
Application & Materials Deadline: March 1, 2022
Individuals who deferred enrollment from 2020 or 2021 may update their application by sending a 1-2 paragraph research progress update to Shannon Silva.

Instructors:

Diana Laird, University of California San Francisco
Francesca Mariani, University of Southern California

Co-Instructors:

Steve Vokes, The University of Texas at Austin
Yojiro Yamanaka, McGill University, Canada

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COVID-19: All participants planning to attend in-person will be required to provide documentary proof of full vaccination AND first booster (when eligible) with an FDA or EMA approved vaccine. Additional safety measures will be in line with current NY and federal guidelines applicable in summer 2022.

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See the roll of honor - who has taken the course in the past.


Follow us on Twitter @cshlmousecourse

This intensive lecture and laboratory course is designed for scientists, typically at the early principal investigator, postdoctoral or graduate stages, interested in using mouse models to study mammalian development, stem cells and cancer. The lecture portion of the course, taught by leaders in the field, provides the conceptual basis for contemporary research in embryogenesis, organogenesis in development and disease, embryonic, adult and induced pluripotent stem cells and cancer biology.

The laboratory and workshop portions of the course provide hands-on introduction to engineering of mouse models, stem cell technologies and tissue analyses. 

Experimental techniques include:

  • CRISPR/Cas9 editing
  • Zygote microinjection and embryo transfer
  • Isolation, culture, manipulation of pre and post-implantation embryos
  • Zygote electroporation and in vivo electroporation
  • Embryo electroporation
  • Embryo roller bottle culture
  • Sperm cryopreservation
  • in vitro fertilization
  • Vibratome and cryosectioning
  • in situ RNA hybridization chain reaction
  • Immunostaining
  • Organ explant culture & fluorescent live imaging
  • Skeletal preparation
  • Transgenic allele design
Confirmed Speakers:

Matt Anderson, National Cancer Institute
Richard Behringer, MD Anderson Cancer Center
Samantha Brugmann, Cincinnati Children's Hospital
Blanche Capel, Duke University
Cristina Cebrián, Cincinnati Children’s Hospital Medical Center
Can Cenik, The University of Texas at Austin
Francesca Cole, MD Anderson Cancer Center
Danelle Devenport, Princeton University
José Antonio Enríquez Domínguez, CNIC, Spain
Mikala Egeblad, Cold Spring Harbor Laboratory
Camilla Forsberg, University of California Santa Cruz
Anna-Katarina Hadjantonakis, Sloan Kettering Institute
Brigid Hogan, Duke University School of Medicine
Marie Kmita, Institut de recherches cliniques de Montréal
Natasza Kurpios, Cornell University College of Veterinary Medicine
Jesse Mager, University of Massachusetts Amherst
Sean Morrison, University of Texas Southwestern Medical School
Steven Munger, The Jackson Laboratory
Paul Trainor, Stowers Institute for Medical Research
George Yancopoulos, Regeneron Pharmaceuticals

Support & Stipends:

Major support provided by: National Cancer Institute.

We would like to acknowledge the following companies that provided invaluable support:

Microscopes: BioTek Instruments, Leica Microsystems Inc, Nikon  Instruments, Inc, Thermo Fisher Scientific
Equipment: Electron Microscopy Sciences, Leica Biosystems, Thermo Fisher Scientific, Sutter Instrument Company, Eppendorf North America, Narishige International USA
Animal Donations: Charles River Laboratories, Taconic Bioscience, Inc, The Jackson Laboratory
Donations: Biolegend, Cosmo Bio Co, Ltd

Stipends are available to offset tuition costs as follows:

       

Please indicate your eligibility for funding in your stipend request submitted when you apply to the course. Stipend requests do not affect selection decisions made by the instructors. 

Cost (including board and lodging): $5,340

No fees are due until you have completed the full application process and are accepted into the course. Students accepted into the course should plan to arrive no later than 5:00pm (EST) on June 7 and plan to depart in the morning on June 27.

Before applying, ensure you have:
  1. Personal statement/essay;
  2. Letter(s) of recommendation;
  3. Curriculum vitae/resume (optional);
  4. Financial aid request (optional).
    More details.

If you are not ready to fully apply but wish to express interest in applying, receive a reminder two weeks prior to the deadline, and tell us about your financial aid requirements, click below: