Expression, Purification & Analysis
of Proteins and Protein Complexes
April 12 -25, 2023
Key Dates
Application Deadline: January 31, 2023
Arrival: April 11th by 6pm EST
Departure: April 25th around 12pm EST
CSHL Courses are intensive, running all day and often including evenings and weekends; students are expected to attend all sessions and reside on campus for the duration of the course.
Instructors:
Michael Marr, Brandeis University
Sergei Nechaev, University of North Dakota School of Medicine
Andrew Quigley, Diamond Light Source, United Kingdom
See the Roll of Honor who's taken the course in the past
This course is for scientists, including graduate students, postdoctoral scholars, staff scientists, and principal investigators, who want a rigorous introduction to expression and purification of proteins as well as analysis of protein structure and function.
Through hands-on experience in the lab as well as extensive lecture and discussion, each student will become familiar with key approaches in expression, purification, and analysis of soluble and membrane proteins and protein complexes from both natural sources and overexpression systems.
The emphasis of the course is on the following:
- Approaches in protein expression: Choosing the best bacterial or eukaryotic expression system tailored for the particular protein and experimental problem; determining how to optimize expression; understanding protein tagging: the advantages and pitfalls of various affinity and solubility tags.
- Approaches in protein purification: Choosing the best strategy for a given protein including solubilization; bulk fractionation; liquid chromatography: including conventional methods (ion exchange, size exclusion, reverse phase, etc.) and affinity methods (e.g., MAC, DNA affinity, immunoaffinity, etc.), as well as FPLC/HPLC.
- Approaches in protein analysis: introduction to common approaches for characterization of proteins including binding assays; activity assays; mass spectroscopy to identify protein interaction partners and post-translational modifications.
In addition to purification, students will also gain exposure to fundamental analytical approaches such as mass spectroscopy and protein structure determination (e.g., X-ray crystallography, cryo-EM, etc.).
2023 Lecturers:
Kate Adamala, University of Minnesota
Don Jarvis, University of Wyoming
Fei Wen, University of Michigan
Denise Okafor, Penn State
Financial aid is available to offset tuition costs as follows:
- Interdisciplinary Fellowships (transitioning from outside biology) & Scholarships (transitioning from other biological disciplines) are provided by the Helmsley Charitable Trust.
- International applicants can apply for financial aid provided by the Howard Hughes Medical Institute.
Please indicate your eligibility for funding in your financial aid request submitted as part of your application materials. Financial aid requests do not affect selection decisions made by the instructors.
We would like to acknowledge the following companies that provided invaluable support
Lab Equipment: Bio-Rad Laboratories, GE Healthcare Corp., Waters
Cost (includes food and housing): $4,210
No fees are due until you have completed the full application process and are accepted into the course.
Before applying, ensure you have (ALL) due by January 31):
- Personal statement/essay;
- Letter(s) of recommendation;
- Curriculum vitae/resume (optional);
- Financial aid request (optional).
More details.
If you are not ready to fully apply but wish to express interest in applying, receive a reminder two weeks prior to the deadline, and tell us about your financial aid requirements, click below:
For more on this course,
read what former trainees said of their experiences. Also, be sure to
check out the growing online alumni presence.