Antibody Engineering & Display Technologies
October 15 - 29, 2024
Application Deadline: July 15, 2024
Arrival: October 15th by 6pm EST
Departure: October 29th around 12pm
CSHL Courses are intensive, running all day and often including evenings and weekends; students are expected to attend all sessions and reside on campus for the duration of the course.
Gregg Silverman, New York University School of Medicine
Gianluca Veggiani, Louisiana State University
Sylvie Briand, University of Zürich, Switzerland
Johannes Yeh, Cold Spring Harbor Laboratory
See the roll of honor - who's taken the course in the past
Advances in the generation and selection of antibodies from combinatorial libraries allow for the rapid production of antibodies from immune and non-immune sources. This intensive laboratory/lecture course will focus on the construction of combinatorial antibody libraries expressed on the surface of filamentous phage and the subsequent selection of desired antibodies from the library. Students will learn the theoretical and practical aspects of constructing combinatorial libraries from immune and non-immune sources as well as the construction of synthetic antibody libraries. Antigen-specific recombinant monoclonal antibodies will be selected from the library. Production, purification and characterization of antibody fragments expressed in E. coli will also be covered.
The lecture series, presented by course faculty and a number of invited speakers, will emphasize theory and practice of antibody display technologies, expression of antibodies in E. coli and mammalian cells, antibody structure and function, bacterial display of antibodies and other ligand-binding domains, the immunobiology of the antibody response, and the use of monoclonal antibodies for therapy including the design of chimeric antigen receptor T cells. We will also discuss principles and protocols for generation and analysis of immune repertoires determined by Next Generation Sequencing.
Prior Year Speakers:
Brandon Dekosky, MIT
Ratmir Derda, University of Alberta, Canada
Mark Howarth, University of Cambridge, United Kingdom
Andreas Pluckthun, University of Zürich, Switzerland
Jasna Rakonjac, Massey University, New Zealand
Sachdev Sidhu, University of Toronto, Canada
Don Siegel, University of Pennsylvania
George Smith, University of Missouri
Stefan Stahl, KTH Royal Institute of Technology, Sweden
Michael Wilson, University of California, San Francisco
Mark Yarmarkovitch, NYU
We would like to acknowledge the following companies that provided invaluable support:
Thermo Fisher Scientific
Support & Stipends
Stipends are available to offset tuition costs as follows:
International applicants (Howard Hughes Medical Institute
Please indicate your eligibility for funding in your stipend request submitted when you apply to the course. Stipend requests do not affect selection decisions made by the instructors.