X-RAY METHODS IN STRUCTURAL BIOLOGY
October 12 - 27, 2015
Application Deadline: June 15, 2015
William Furey, V.A. Medical Center, Pittsburgh
Gary Gilliland, Janssen Research & Development LLC
Alexander McPherson, University of California, Irvine
James Pflugrath, Rigaku Americas Corporation, Texas
See the Roll of Honor - who's taken the course in the past
Crystallography and X-ray diffraction yield a wealth of structural information unobtainable through other methods. This intense laboratory/computational course focuses on the major techniques used to determine the three-dimensional structures of macromolecules. It is designed for scientists with a working knowledge of protein structure and function, but who are new to macromolecular crystallography. Topics to be covered include basic diffraction theory, crystallization (proteins, nucleic acids and complexes), crystal characterization, X-ray sources and optics, synchrotrons, Cryocrystallography, data collection, data reduction, heavy atom substructure determination, multiple isomorphous replacement, single and multiwavelength anomalous diffraction, molecular replacement, solvent flattening, non-crystallographic symmetry averaging, electron density interpretation, molecular graphics, structure refinement, structure validation, coordinate deposition and structure presentation.
Participants learn through extensive hands-on experiments in fully equipped labs. Participants will crystallize multiple proteins and determine their crystal structures by several methods while learning through extensive lectures on Crystallographic theory and informal discussions behind the techniques. Applicants should be familiar with the creation and editing of simple text files on Linux workstations using a screen based editor (either vi or emacs).
Speakers in the 2014 course included:
Paul Adams, Dominika Borek, Martin Caffrey, Paul Emsley, Wayne Hendrickson, James Holton, Ryan Jackson, Xinhua Ji, Leemor Joshua-Tor, Gerard Kleywegt, Anastassis Perrakis, Randy Read, David Richardson, Clyde Smith, Robert Sweet, Thomas Terwilliger, Andrea Thorn, John Westbrook
This course is supported with funds provided by the National Institute of General Medical Sciences
Cost (including board and lodging): $4,080
This button links to a short form which confirms your interest in the course.
No fees are due until you have completed the full application process and
are accepted into the course.
Students accepted into the course should plan to arrive by early evening on October 11 and plan to depart after lunch on October 27.