June 30 -July 16, 2015
Application Deadline: March 31, 2015

Cary Lai,
University of Indiana
Joseph Loturco, University of Connecticut
Anne Schaefer, Icahn School of Medicine, Mount Sinai Hospital

See the roll of honor - who's taken the course in the past

This laboratory and lecture course is designed to provide neuroscientists at all levels with a conceptual and practical understanding of several of the most advanced techniques in molecular neuroscience. The course curriculum is divided into three sections: an extensive and up-to-date set of laboratory exercises, daily lectures covering the theoretical and practical aspects of the various methods used in the laboratory, and a series of evening research seminars. The informal and interactive evening lectures will be given by leading molecular neuroscientists and will serve to illustrate the ways in which the various experimental approaches have been used to advance specific areas of neurobiology. In this year's course, the laboratory portion will include topics such as: an introduction to the design and use of animal virus vectors in neurobiology; the use of CRISPR genome editing and RNAi approaches for regulating the expression of specific genes in neurons; practical exercises in gene delivery systems including mammalian cell infection and transfection and electroporation techniques for targeted gene transfer in vivo; an introduction to overall strategies, use and design of BAC transgenic vectors; real time RT-PCR analyses; assays of chromatin and chromatin structure in neurons, and mRNA isolation from specified neural subtypes by TRAP.
2015 Speakers :
Ben Barres, Stanford University School of Medicine
Emiliana Borrelli, University of California, Irvine
Dawen Cai, University of Michigan Medical School
Le Cong, Broad Institute of MIT and Harvard
Robert Darnell, The Rockefeller University
Joseph Dougherty, Washington University
James Eberwine, University of Pennsylvania Perelman School of Medicine
Viviana Gradinaru, California Institute of Technology
Kurt Haas, University of British Columbia, Canada
Oliver Hobert, Columbia University College of P&S
Arnold Kriegstein, University of California, San Francisco
Bryan Luikart, Geisel School of Medicine at Dartmouth
Brady Maher, Lieber Institute, Johns Hopkins Medical Institute
Craig Nelson, University of Connecticut

The CSHL Meetings & Courses Program is strongly committed to diversity in the recruitment and selection of participants for all our courses. See our diversity policy here. We strongly encourage individuals from diverse backgrounds to apply for this course.

This course may be supported with major funding provided by: National Institute of Mental Health and Howard Hughes Medical Institute.

Equipment and reagents generously provided by: New England Biolabs Inc.; Olympus America Inc.; Carl Zeiss Inc. along with Bitplane-Andor Technology; Bio-Rad Laboratories; Chroma Technology Corporation; Coherent Laser; Eppendorf North America; Fotodyne Inc.; Harvard Apparatus Inc.; Illumina Inc.; Invitrogen Corporation; Leica Microsystems Inc.; Nanodrop Technologies; Narishige International USA Inc.; PerkinElmer Life; Promega Corporation; Qiagen Inc.; Roche Applied Science; Sigma-Aldrich; Thermo Fisher Scientific.

Scientists from developing countries accepted into this course may be eligible for scholarships provided by the International Brain Research Organization 
Cost (including board and lodging): $4,080

This button links to a short form which confirms your interest in the course.
No fees are due until you have completed the full application process and
are accepted into the course.


Students accepted into the course should plan to arrive by early evening on June 29 and plan to depart after lunch on June 16.