Cell & Developmental Biology of Xenopus:
Gene Discovery & Disease
April 4 - 17, 2018
Application Deadline: January 31, 2018

Instructors:

Mustafa Khokha, Yale University
Karen Liu, King's College London, UK

See the roll of honor - who's taken the course in the past

In vivo animal models are an important tool for the understanding of human development and disease. Studies using the frog Xenopus have made remarkable contributions to our understanding of fundamental processes such as cell cycle regulation,transcription, translation and many other topics. Xenopus is remarkable for studying development and disease,including birth defects, cancer, and stem cell biology. Because Xenopus are easy to raise, producing many thousands of eggs per day, these frogs have emerged as a premiere model for understanding of human biology from the fundamental building blocks to the whole organism.

The recent development of CRISPR/Cas9 technology has made it easy to target genes of interest using Xenopus. This course has been designed with that in mind. Our goal is for each student to design a set of experiments focusing on their gene or biological interest.Prior to starting the course, students will be expected to choose gene(s) of interest, and the instructors will generate sgRNAs targeting these genes. These can be the students’ own genes, or chosen from a bank provided by the instructors.

During the course, the students will analyze any phenotypes generated from CRISPR/Cas9 based gene depletion while learning the diverse array of techniques available in Xenopus. In previous courses, we have guided students in the ablation of a wide variety of genes and helped them design suitable assays for their biological interests. Most recently, students have targeted autism genes, thyroid genes and immune modulators, several of which have already led to publications. Approaches covered will include microinjection and molecular manipulations such as CRISPR/Cas9 knockouts, antisense morpholino-based depletions, transgenics, and mRNA overexpression. In addition, students can combine these techniques with explant and transplant methods to simplify or test tissue level interactions. Additional methods include mRNA in situ hybridization and protein immunohistochemistry as well as basic bioinformatic techniques for gene comparison and functional analysis. Biochemical approaches such as proteomics and mass spectrometry and biomechanical concepts will also be discussed. Finally, to visualize subcellular and intercellular activities, we will introduce a variety of imaging methods including time-lapse, fluorescent imaging, optical coherence tomography and confocal microscopy. These are facilitated by state-of-the-art equipment from Nikon, Leica, Thorlabs, and Bruker.

Due to the tailored nature of this course, it is suitable for those new to the Xenopus field, as well as for more advanced students who are interested in emerging technologies. Please feel free to contact the instructors for informal guidance.

Past 2017 Lecturers:

Ira Blitz, University of California, Irvine
Sang-Wook Cha, Cincinnati Children's Hospital Medical Center
Frank Conlon, University of North Carolina
Lance Davidson, University of Pittsburgh
Rebecca Heald, University of California, Berkeley
Raymond Keller, University of Virginia
Roberto Mayor, University College London, UK
Ann Miller, University of Michigan
Peter Nemes, George Washington University
Benjamin Steventon, University of Cambridge, UK
Gerald Thomsen, Stony Brook University
John Wallingford, University of Texas at Austin
Andrea Wills, University of Washington



10fps of Xenopus GRP cilia

10 frames per second imaging of Xenopus GRP cilia. GFP labeled cilia, RFP membrane marker. Courtesy of Melanie Tingler, Shiaulou Yuan, and Mustafa Khokha. Cold Spring Harbor Xenopus Course, Yale University. Movie generated on Bruker Opterra II confocal microscope.

125 fps blood flow

125 frames per second imaging of Xenopus red blood cells in gills. Playback at 5x slow motion. Video courtesy of Vaughn Colleluori and Mustafa Khokha, Cold Spring Harbor Xenopus Course,Yale University. Movie generated on Bruker Opterra II confocal microscope.

CLAMP GFP and RFP

CLAMP GFP (green) marks tips of cilia and membrane RFP (red) marks cilia axoneme. Two color images collected at 20 fps. CSHL 2015 Xenopus course. Movie generated on Bruker Opterra II confocal microscope.


Support & Stipends

Major support provided by the National Institute of Child Health and Human Development

Stipends are available to offset tuition costs as follows:

       

US applicants (National Institute of Child Health and Human Development )
Interdisciplinary Fellowships (transitioning from outside biology)  & Scholarships (transitioning from other biological disciplines) (Helmsley Charitable Trust)
International applicants (Howard Hughes Medical Institute)

Please indicate your eligibility for funding in your stipend request submitted when you apply to the course. Stipend requests do not affect selection decisions made by the instructors. 

Cost (including board and lodging): $3,885

This button links to a short form which confirms your interest in the course. No fees are due until you have completed the full application process and are accepted into the course.

Students accepted into the course should plan to arrive by early evening on April 3 and plan to depart after lunch on April 17.