Jennifer Waters, Harvard Medical School
Beth Cimini, Broad Institute of MIT and Harvard, Cambridge, MA
Florian Jug, Fondazione Human Technopole, Italy
Hunter Elliott, Bighat Biosciences
Talley Lambert, Harvard Medical School
We provide a thorough treatment of the complete process of quantitative imaging, from the photons emitted from the sample to the extraction of biologically meaningful measurements from digital images. Material is covered in lectures, discussion groups and hands-on quantitative exercises using commercial microscopes and open-source image analysis tools.
Concepts Covered Include:
- Widefield fluorescence microscopy
- Laser scanning and spinning disk confocal microscopy
- CCD, EM-CCD & sCMOS cameras
- Total internal reflection fluorescence microscopy (TIRF)
- Light sheet microscopy
- Super-resolution microscopy (structured illumination, STED & localization microscopy)
- Imaging and analyzing ratiometric “biosensors” (including FRET)
- Fluorescent proteins and live sample imaging
- Image processing (filtering, de-noising, corrections, deconvolution)
- Image segmentation
- Quantitative shape and intensity measurements
- Object detection and tracking
- Machine learning
- Designing and troubleshooting quantitative imaging experiments
- ...and more!
The course will also include a series of seminars from guest speakers who apply the methods we will discuss.
2024 Teaching Assistants
Damian Dalle Nogare, Fondazione Human Technopole, Milano, Italy
Eva de la Serna, Harvard Medical School, ,
Federico Gasparoli, Harvard Medical School, Boston, MA
Anna Jost, Harvard Medical School, Boston, MA
2024 Invited Speaker
Chad Hobson, NEW Affiliation, Chapel Hill, NC
Support & Stipends
Major support for this course is provided by the National Cancer Institute.
This course is supported in part by grants from Howard Hughes Medical Institute through the Science Education Program.
We would like to acknowledge the following companies that provided invaluable support:
Microscopes: 89 North, Abberior, Applied Scientific Instrumentation, Biovision Technologies, Inc., Leica Microsystems, Nikon Instruments, Prior Scientific, Scientifica
Cameras: Hamamatsu Photonic Systems, PCO, Photometrics
Filters and Filter Cubes: Chroma Technology
Incubation: Okolab, Tokai Hit USA, Inc.
Lasers: Coherent, Toptica Photonics Inc.
Light Sources: Lumencor
Software: Andor Technology
Financial aid is available to help offset tuition costs as follows:
Please indicate your eligibility for funding in your financial aid request submitted as part of your application materials. Financial aid requests do not affect selection decisions made by the instructors.
Cost (includes food and housing): $4,645 USD
No fees are due until you have completed the full application process and are accepted into the course.
Before applying, ensure you have:
- Personal statement/essay;
- Two letters of recommendation;
- Curriculum vitae/resume;
- Financial aid request (optional).
More details.
Your statement should describe your current research project, with an emphasis on planned or current quantitative microscopy experiments, a list of the microscopes to which you have access to use for your research and what you hope to gain by participating in the course.
If you are not ready to fully apply but wish to express interest in applying, receive a reminder two weeks prior to the deadline, and tell us about your financial aid requirements, click below: