Expression, Purification & Analysis
of Proteins and Protein Complexes
April 4 - 17, 2018
Application Deadline: January 31, 2018

Instructors:

Albert Courey, University of California, Los Angeles
Sergei Nechaev, University of North Dakota School of Medicine
Michael Marr, Brandeis University
Sue-Hwa Lin, University of Texas M.D. Anderson Cancer Center

See the roll of honor - who's taken the course in the past

This course is for scientists, including graduate students, postdoctoral scholars, staff scientists, and principal investigators, who want a rigorous introduction to expression and purification of proteins as well as analysis of protein structure and function.

Through hands-on experience in the lab as well as extensive lecture and discussion, each student will become familiar with key approaches in expression, purification, and analysis of soluble and membrane proteins and protein complexes from both natural sources and overexpression systems. The emphasis of the course is on the following:

1) Approaches in protein expression: Choosing the best bacterial or eukaryotic expression system tailored for the particular protein and experimental problem; determining how to optimize expression; understanding protein tagging: the advantages and pitfalls of various affinity and solubility tags.

2) Approaches in protein purification: Choosing the best strategy for a given protein including solubilization; bulk fractionation; liquid chromatography: including conventional methods (ion exchange, size exclusion, reverse phase, etc.) and affinity methods (e.g., MAC, DNA affinity, immunoaffinity, etc.), as well as FPLC/HPLC.

3) Approaches in protein analysis: introduction to common approaches for characterization of proteins including binding assays; activity assays; mass spectroscopy to identify protein interaction partners and post-translational modifications.

In addition to purification, students will also gain exposure to fundamental analytical approaches such as mass spectroscopy and protein structure determination (e.g., X-ray crystallography, cryo-EM, etc.).

Confirmed 2017 Lecturers:

Richard Burgess, University of Wisconsin, Madison
Jiansen Jiang, University of California, Los Angeles
James Love, ATUM
Darryl Pappin, Cold Spring Harbor Laboratory

Support & Stipends

Major support provided by the National Cancer Institute

Stipends are available to offset tuition costs as follows:

       

US applicants (National Cancer Institute)
Interdisciplinary Fellowships (transitioning from outside biology)  & Scholarships (transitioning from other biological disciplines) (Helmsley Charitable Trust)
International applicants (Howard Hughes Medical Institute)

Please indicate your eligibility for funding in your stipend request submitted when you apply to the course. Stipend requests do not affect selection decisions made by the instructors. 

Cost (including board and lodging): $3,885

This button links to a short form which confirms your interest in the course. No fees are due until you have completed the full application process and are accepted into the course.

Students accepted into the course should plan to arrive by early evening on April 3 and plan to depart after lunch on April 17.