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Mouse Development, Stem Cells & Cancer (CANCELED)
June 3 - 22, 2020
Application & Materials Deadline: March 1, 2020


Instructors:

Tamara Caspary, Emory University School of Medicine
Camilla Forsberg, University of California Santa Cruz 

Co-Instructors:

Diana Laird, University of California San Francisco
Francesca Mariani, University of Southern California

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COVID-19: UPDATE March 24, 2020: It is with considerable regret that we announce the cancellation of this course for 2020 but it will be rescheduled to similar dates in 2021.

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See the roll of honor - who has taken the course in the past.

This intensive lecture and laboratory course is designed for scientists, typically at the early principal investigator, postdoctoral or graduate stages, interested in using mouse models to study mammalian development, stem cells and cancer. The lecture portion of the course, taught by leaders in the field, provides the conceptual basis for contemporary research in embryogenesis, organogenesis in development and disease, embryonic, adult and induced pluripotent stem cells and cancer biology.

The laboratory and workshop portions of the course provide hands-on introduction to engineering of mouse models, stem cell technologies and tissue analyses. 


Experimental techniques include:

  • CRISPR/Cas9 in embryos

  • Zygote microinjection and embryo transfer

  • Isolation, culture, manipulation of pre and post-implantation embryos

  • Embryo electroporation

  • Embryo roller bottle culture

  • Sperm cryopreservation

  • in vitro fertilization

  • Culture of mouse embryonic stem cells and fibroblasts

  • Synthetic embryo generation

  • Vibratome and cryosectioning

  • in situ RNA hybridization chain reaction

  • Immunostaining

  • Multicolor flow cytometry

  • Organ explant culture & fluorescent live imaging

  • Skeletal preparation

  • Hematopoietic stem cell transplant


2020 Speakers Include:

Benjamin Allen, University of Michigan
Matt Anderson, National Cancer Institute
Maria Barna, Stanford University
Richard Behringer, MD Anderson Cancer Center
Blanche Capel, Duke University
Cristina Cebrián, Cincinnati Children’s Hospital Medical Center
Francesca Cole, MD Anderson Cancer Center
José Antonio Enríquez Domínguez, Centro Nacional de Investigaciónes Cardiovasculares Carlos III, Spain
Anna-Katarina Hadjantonakis, Sloan Kettering Institute
Brigid Hogan, Duke University School of Medicine
Anne Ferguson-Smith, University of Cambridge, United Kingdom
Scott Fraser, University of Southern California
Jesse Mager, University of Massachusetts, Amherst
Sean Morrison, University of Texas Southwestern Medical School
Steven Munger, The Jackson Laboratory
Nadia Rosenthal, The Jackson Laboratory 
Paul Trainor, Stowers Institute for Medical Research
Yojiro Yamanaka, McGill University, Canada
George Yancopoulos, Regeneron Pharmaceuticals


Support & Stipends:

Major support provided by: National Cancer Institute.

We would like to acknowledge the following companies that provided invaluable support:
Microscopes:
BioTek Instruments, Leica Microsystems Inc, Nikon  Instruments, Inc, Thermo Fisher Scientific
Equipment: Electron Microscopy Sciences, Leica Biosystems, Thermo Fisher Scientific, Sutter Instrument Company, Eppendorf North America, Narishige International USA
Animal Donations: Charles River Laboratories, Taconic Bioscience, Inc, The Jackson Laboratory
Donations: Biolegend, Cosmo Bio Co, Ltd

Stipends are available to offset tuition costs as follows:

       

Please indicate your eligibility for funding in your stipend request submitted when you apply to the course. Stipend requests do not affect selection decisions made by the instructors. 

Cost (including board and lodging): $5,140

No fees are due until you have completed the full application process and are accepted into the course. Students accepted into the course should plan to arrive no later than 5:00pm (EST) on June 2 and plan to depart in the morning on June 22.

Before applying, ensure you have:
  1. Personal statement/essay;
  2. Letter(s) of recommendation;
  3. Curriculum vitae/resume (optional);
  4. Financial aid request (optional).
    More details.

If you are not ready to fully apply but wish to express interest in applying, receive a reminder two weeks prior to the deadline, and tell us about your financial aid requirements, click below: