This intensive lecture and laboratory course is designed for scientists interested in using mouse models to study mammalian development, stem cells and cancer. The lecture portion of the course, taught by leaders in the field, provides the conceptual basis for contemporary research in embryogenesis, organogenesis in development and disease, embryonic, adult and induced pluripotent stem cells and cancer biology.

The laboratory and workshop portions of the course provide hands-on introduction to engineering of mouse models, stem cell technologies and tissue analyses. Experimental techniques include genome editing by CRISPR/Cas9, Ex vivo and in vivo embryo genome editing usingadeno-associated viruses, pronuclear microinjection, isolation and culture/manipulation of pre- and post-implantation embryos, embryo transfer, embryo electroporation and roller bottle culture, chimera generation, generation and differentiation of mouse embryonic stem cells and fibroblasts, vibratome and cryosectioning, in situ RNA hybridization, immunostaining, FACS sorting and analysis of hematopoietic stem cells, skeletal preparation, organ explant culture and fluorescent imaging, including live time-lapse microscopy.

Support & Stipends

Major support provided by the National Cancer Institute

Stipends are available to offset tuition costs as follows:

US applicants (National Cancer Institute)
Interdisciplinary Fellowships (transitioning from outside biology)  & Scholarships (transitioning from other biological disciplines) (Helmsley Charitable Trust)
International applicants (Howard Hughes Medical Institute)

Please indicate your eligibility for funding in your stipend request submitted when you apply to the course. Stipend requests do not affect selection decisions made by the instructors.