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Mouse Development and Disease Modeling
June 7 - 27, 2027

Key Dates
Application Deadline: March 5, 2027
Arrival: June 7th, by 6pm
Departure: June 27th, around 12pm EST

CSHL Courses are intensive, running all day and often including evenings and weekends; students are expected to attend all sessions and reside on campus for the duration of the course. The programs are not offered for academic credit or completion of an academic program of study in the U.S.

Instructors
Matthew Anderson, NCI-Frederick, Frederick, MD 
Katherine Fantauzzo, University of Colorado Anschutz Medical Campus, Aurora, CO 

Co-Instructors
Ripla Arora, Michigan State University, East Lansing, MI 
Eszter Posfai, Princeton University, Princeton, United States 

See the roll of honor - who has taken the course in the past.

Follow us on Twitter @cshlmousecourse

This lecture and laboratory course is designed for scientists,typically at the early principal investigator, postdoctoral or graduate stages,interested in using mouse models to study mammalian development and disease.The lecture portion of the course, taught by leaders in the field, provides theconceptual basis for contemporary research in embryogenesis, organogenesis, andmodels of developmental and adult diseases.

The laboratory and workshop portions of the course providehands-on introduction to engineering of mouse models, and analysis of modelsutilizing both classic and modern techniques. 

Experimental techniques include

  • Collection of oocytes and sperm
  • In vitro fertilization
  • CRISPR/Cas9 allele design and electroporation
  • Non-surgical embryo transfer
  • Blastocyst flushing and outgrowth
  • iGONAD
  • Dissection of post-implantation embryos 
  • Embryo roller bottle culture 
  • Hybridization chain reaction in situ 
  • Mouse embryonic fibroblast cell culture
  • Cryosectioning and immunofluorescence analysis
  • Organ culture
  • Live imaging and confocal microscopy
  • Skeletal preparations 

60x composite fluorescence micrograph
E14.5 mouse brain section
Fluorescently labeled mouse embryo
Fluorescence micrograph of lung tissue

Prior Year Invited Speakers

Ripla Arora, Michigan State University, East Lansing, MI
Richard Behringer, University of Texas MD Anderson Cancer Center, Houston, TX
Jeffrey Bush, University of California, San Francisco, San Francisco, CA
Katie Cockburn, McGill University, Montreal, Canada
Anna-Katerina Hadjantonakis, Sloan Kettering Institute, New York, NY
Diana Laird, University of California, San Francisco, San Francisco, CA
Ai Ing Lim, Princeton University, Princeton, NJ
Francesca Mariani, University of Southern California, Los Angeles, CA
Kate McDole, MRC Laboratory of Molecular Biology, Cambridge, United Kingdom
Steve Murray, The Jackson Laboratory, Bar Harbor, MD
Virginia Papaioannou, Columbia University Medical Center, New York, NY
Eszter Posfai, Princeton University, Princeton, NJ
Pedro Rocha, National Institutes of Health, Bethesda, MD
Shyam Sharan, National Cancer Institute, NIH, Frederick, MD
Berna Sozen, Yale University, New Haven, CT
Shankar Srinivas, University of Oxford, Oxford, United Kingdom
Rolf Stottmann, Nationwide Children's Hospital Medical Center, Columbus, OH
Kaelyn Sumigray, Yale School of Medicine, New Haven, CT
Xin Sun, University of California, San Diego, La Jolla, CA
Paul Trainor, Stowers Institute for Medical Research, Kansas City
Steven Vokes, The University of Texas at Austin, Austin
Yojiro Yamanaka, Goodman Cancer Institute, McGill University, Montreal, Canada 

Support & Stipends:

Major support provided by the National Cancer Institute of the National Institutes of Health.

We would like to acknowledge the following companies that provided invaluable support:
Agilent Technologies, BEX Co, Ltd., Calibre Scientific, Charles River Laboratories, Epredia, Leica Biosystems Inc., Leica Microsystems Inc. Narishige International USA, Inc., Nikon Instruments Inc., Sutter Instrument, and Tomocube USA

We would like to thank the following company for their generous donation:
JAX/The Jackson Laboratory

Stipends are available to offset tuition costs.

Please indicate your eligibility for funding in your stipend request submitted when you apply to the course. Stipend requests do not affect selection decisions made by the instructors. 

Cost (including board and lodging): $6,255 USD

No fees are due until you have completed the full application process and are accepted into the course. 

Before applying, ensure you have:
  1. Personal statement/essay;
  2. Letter(s) of recommendation;
  3. Curriculum vitae/resume (optional);
  4. Financial aid request (optional).  Stipend requests do not affect selection decisions by the Instructors.
    More details.

If you are not ready to fully apply but wish to express interest in applying, receive a reminder two weeks prior to the deadline, and tell us about your financial aid requirements, click below: