High Throughput Neuroanatomy
October 20 - 31, 2023
Application Deadline: July 15, 2023
Arrival: October 19th by 6pm ESTDeparture: October 31st around 12pm
CSHL Courses are intensive, running all day and often including evenings and weekends; students are expected to attend all sessions and reside on campus for the duration of the course.
Xiaoyin Chen, Allen Institute
Laura DeNardo, University of California Los Angeles
Justus Kebschull, Johns Hopkins University
Lindsay Schwarz, St. Jude Children's Research Hospital
COVID19: All participants planning to attend in-person will be required to attest to recent COVID vaccination (within one year of the course’s start date) with an FDA or WHO approved vaccine. Additional safety measures will be in line with current NY and Federal Guidelines applicable in Fall 2023.
Modern high-throughput neuroanatomical tools—including but not limited to barcode-based tracing methods—allow unprecedented insight into the structure of the nervous system. Their use, however, is currently restricted to small set of expert laboratories. This limited uptake is not due to an inherent cost or difficulty of these techniques, but rather due to a lack of training opportunities. In this new CSHL course, we aim to teach the core skills required for successfully applying a range of modern neuroanatomical techniques. We will cover viral tracing tools, including anterograde, retrograde, and transsynaptic tracers, brain clearing and whole brain analysis of axonal data, MAPseq and BARseq, as well as RNA in situ sequencing technologies. In practicals, students will learn how to prepare virus, how to acquire and analyze whole-brain volumetric axon tracing data, and how to perform a BARseq2 experiment. After completing this course, we expect students to have the skills to implement a range of cutting-edge neuroanatomical techniques in their own labs.
2022 Speakers Included:
Edward Boyden, Massachusetts Institute of Technology/HHMI
Edward Callaway, The Salk Institute for Biological Studies
Ali Ertürk, Helmholtz Zentrum, Munich, Germany
Liqun Luo, Stanford University
Emily Sylwestrak, University of Oregon
Bosiljka Tasic, Allen Institute for Brain Science
Xiao Wang, Broad Institute/MIT
Tony Zador, Cold Spring Harbor Laboratory
Hongkui Zeng, Allen Institute for Brain Science
Support & Stipends
We would like to acknowledge the following companies for equipment loans and technical support:
Equipment: Leica Biosystems, Miltenyi Biotec, Nikon Instruments
Stipends are available to offset tuition costs as follows:
Please indicate your eligibility for funding in your stipend request submitted when you apply to the course. Stipend requests do not affect selection decisions made by the instructors.
Cost (including board and lodging): $3,890
No fees are due until you have completed the full application process and are accepted into the course.
Before applying, ensure you have (all due July 15):
- Personal statement/essay;
- Letter(s) of recommendation;
- Curriculum vitae/resume (optional);
- Financial aid request (optional).
If you are not ready to fully apply but wish to express interest in applying, receive a reminder two weeks prior to the deadline, and tell us about your financial aid requirements, click below: