Meetings & Courses Program

Crossroads of Biology

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High Throughput Neuroanatomy
October 16 - 28 2025

Key Dates:
Application Deadline:  July 15, 2025
Arrival: October 16th by 6pm EST
Departure: October 28th around 12pm 

CSHL Courses are intensive, running all day and often including evenings and weekends; students are expected to attend all sessions and reside on campus for the duration of the course.

Instructors:
Cathryn Cadwell, University of California, San Francisco, San Francisco, SC 
Xiaoyin Chen, Allen Institute for Brain Science
Laura DeNardo, University of California Los Angeles
Justus Kebschull, Johns Hopkins University

See the roll of honor - who's taken the course in the past.

Modern high-throughput neuroanatomical tools—including but not limited to barcode-based tracing methods—allow unprecedented insight into the structure of the nervous system. Their use, however, is currently restricted to small set of expert laboratories. This limited uptake is not due to an inherent cost or difficulty of these techniques, but rather due to a lack of training opportunities. In this new CSHL course, we aim to teach the core skills required for successfully applying a range of modern neuroanatomical techniques. We will cover viral tracing tools, including anterograde, retrograde, and transsynaptic tracers, brain clearing and whole brain analysis of axonal data, MAPseq and BARseq, as well as RNA in situ sequencing technologies. In practicals, students will learn how to prepare virus, how to acquire and analyze whole-brain volumetric axon tracing data, and how to perform a BARseq2 experiment. After completing this course, we expect students to have the skills to implement a range of cutting-edge neuroanatomical techniques in their own labs.

2025 Invited Speakers
Edward Boyden, MIT, HHMI, Cambridge, MA 
Elizabeth Hillman, Columbia University / St. Jude Hospital, New York, NY 
Xin Jin, Scripps Research, La Jolla, CA 
Gilles Laurent, Max-Planck-Institute for Brain Research, Frankfurt Am Main, Germany 
Jeantine Lunshof, Harvard University, Boston, MA 
Andrew Payne, E11 Bio, Alameda, CA 
Jing Ren, MRC Laboratory of Molecular Biology, Cambridge, United Kingdom 
Lindsay Schwarz, St. Jude Children's Research Hospital, Memphis, TN 
Nenad Sestan, Yale University Medical School, New Haven, CT 
Bosiljka Tasic, Allen Institute, Seattle, WA 
Xiao Wang, MIT and Broad Institute, Cambridge, MA 
Anthony Zador, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 


Support & Stipends

We would like to acknowledge the following companies for equipment loans and technical support:
Equipment: Leica Biosystems, Miltenyi Biotec, Nikon Instruments

Stipends are available to offset tuition costs as follows:

        

Please indicate your eligibility for funding in your stipend request submitted when you apply to the course. Stipend requests do not affect selection decisions made by the instructors.

Cost (including board and lodging): $4,295 USD

No fees are due until you have completed the full application process and are accepted into the course. 

Before applying, ensure you have (all due July 15):
  1. Personal statement/essay;
  2. Letter(s) of recommendation;
  3. Curriculum vitae/resume (optional);
  4. Financial aid request (optional).
    More details.

If you are not ready to fully apply but wish to express interest in applying, receive a reminder two weeks prior to the deadline, and tell us about your financial aid requirements, click below: