Cell & Developmental Biology of Xenopus:
Gene Discovery & Disease
April 5 - 18, 2016
Application Deadline: February 21, 2016
Mustafa Khokha, Yale University
Karen Liu, King's College London, UK
Techniques covered in this course will include microinjection, molecular manipulations such as CRISPR knockouts, transgenics, and mRNA overexpression. These can be combined with explant and transplant methods to simplify or test tissue level interactions. To visualize subcellular and intercellular activities, we will introduce a variety of imaging methods including time-lapse, fluorescent and confocal microscopy. Additional methods include mRNA in situ hybridization and protein immunohistochemistry as well as basic bioinformatic techniques for gene comparison and functional analysis.
This course is designed for those new to the Xenopus field, as well as for more advanced students who are interested in emerging technologies. We encourage students to bring their own genes of interest and will tailor aspects of the course to enable them to initiate studies on their specific projects.
Ira Blitz, University of California, Irvine
Sang-Wook Cha, Cincinnati Childrens Hospital Medical Center
Frank Conlon, University of North Carolina
Helene Cousin, University of Massachusetts, Amherst
Lance Davidson, University of Pittsburgh
Heithem El-Hodiri, Nationwide Childrens Hospital Research Institute
Rebecca Heald, University of California, Berkeley
Raymond Keller, University of Virginia
Ann Miller, University of Michigan
Gerald Thomsen, Stony Brook University
John Wallingford, University of Texas at Austin
Andrea Wills, Stanford University
Support & Stipends
Major support provided by the National Institute of Child Health and Human Development
Stipends are available to offset tuition costs as follows:
US applicants (National Institute of Child Health and Human Development )
Interdisciplinary Fellowships (transitioning from outside biology) & Scholarships (transitioning from other biological disciplines) (Helmsley Charitable Trust)
International applicants (Howard Hughes Medical Institute)
Please indicate your eligibility for funding in your stipend request submitted when you apply to the course. Stipend requests do not affect selection decisions made by the instructors.
Cost (including board and lodging): $3,735
This button links to a short form which confirms your interest in the course. No fees are due until you have completed the full application process and are accepted into the course.
Students accepted into the course should plan to arrive by early evening on April 4 and plan to depart after lunch on April 18.