Mouse Development, Stem Cells & Cancer
June 5 - 24, 2019
Application Deadline: March 1, 2019
Instructors:
Tamara Caspary, Emory University School of Medicine
Camilla Forsberg, University of California Santa Cruz
Co-Instructors:
Diana Laird, University of California San Francisco
Francesca Mariani, University of Southern California
This intensive lecture and laboratory course is designed for scientists interested in using mouse models to study mammalian development, stem cells and cancer. The lecture portion of the course, taught by leaders in the field, provides the conceptual basis for contemporary research in embryogenesis, organogenesis in development and disease, embryonic, adult and induced pluripotent stem cells and cancer biology.
The laboratory and workshop portions of the course provide hands-on introduction to engineering of mouse models, stem cell technologies and tissue analyses.
Experimental techniques include:
- Genome editing by CRISPR/Cas9
- Zygote microinjection
- Isolation and culture/manipulation of pre- and post-implantation embryos
- Embryo transfer
- Embryo electroporation
- Roller bottle culture
- Sperm cryopreservation
- in vitro fertilization
- Culture of mouse embryonic stem cells and fibroblasts
- Synthetic embryo generation
- Vibratome and cryosectioning
- in situ RNA hybridization
- Immunostaining
- FACS sorting
- Analysis of hematopoietic stem cells, skeletal preparation, organ explant culture and fluorescent imaging, including live time-lapse microscopy.
2019 Speakers:
Kim Cooper, University of California San Diego
Frank Costantini, Columbia University
Caroline Dive, University of Manchester, United Kingdom
Brigid Hogan, Duke University School of Medicine
Monica Justice, University of Toronto, Canada
Mark Lewandoski, NIH
Jesse Mager, University of Massachusetts, Amherst
Helen McNeill, Washington University
Melissa Reeves, University of California San Francisco
Tristan Rodriguez, Imperial College London, United Kingdom
Maike Sander, University of California San Diego
Michael Shen, Columbia University
Phillipe Soriano, Mount Sinai School of Medicine
Patrick Tam, University of Sydney School of Medicine, Australia
Paul Trainor, Stowers Institute for Medical Research
Steven Vokes, University of Texas Austin
Yojiro Yamanaka, McGill Cancer Research Centre, Canada
Magdalena Zernicka-Goetz, University of Cambridge, United Kingdom
Support & Stipends:
Major support provided by: National Cancer Institute.
Stipends are available to offset tuition costs as follows-
Please indicate your eligibility for funding in your stipend request submitted when you apply to the course. Stipend requests do not affect selection decisions made by the instructors.
We would like to acknowledge the following companies that provided invaluable support:
Microscopes: BioTek Instruments, Leica Microsystems, Inc., Nikon Instruments, Inc., Thermo Fisher Scientific
Equipment: Electron Microscopy Sciences, Leica Biosystems, Thermo Fisher Scientific, Sutter Instrument Company, Eppendorf North America, Narishige International USA
Animal Donations: Charles River Laboratories, Taconic Biosciences, Inc., The Jackson Laboratory
Donations: Biolegend, Cosmo Bio Co., LTD
Cost (including board and lodging): $4,990
No fees are due until you have completed the full application process and are accepted into the course. Students accepted into the course should plan to arrive no later than 5:00pm (EST) on Tuesday June 4 and plan to depart in the morning on June 24.
Before applying, ensure you have:
- Personal statement/essay;
- Letter(s) of recommendation;
- Curriculum vitae/resume (optional);
- Financial aid request (optional).
More details.
If you are not ready to fully apply but wish to express interest in applying, receive a reminder two weeks prior to the deadline, and tell us about your financial aid requirements, click below: