MOUSE DEVELOPMENT, STEM CELLS & CANCER
June 3 - 23, 2015
Application Deadline: March 15, 2015

Instructors:
Mark Lewandoski, National Cancer Center/NIH
Deneen Wellik, University of Michigan Medical Center

Co-Instructors:
Amy Ralston, Michigan State University
Licia Selleri, Weill Cornell Medical College
 
See the roll of honor - who's taken the course in the past

This intensive lecture and laboratory course is designed for scientists interested in using mouse models to study mammalian development, stem cells and cancer. The lecture portion of the course, taught by leaders in the field, provides the conceptual basis for contemporary research in embryogenesis, mathematical modeling of disease and development, organogenesis, embryonic, adult and induced pluripotent stem cells and cancer biology.

The laboratory and workshop portions of the course provides hands-on introduction to engineering of mouse models, stem cell technologies and tissue analyses. Experimental techniques include genome editing by CRISPR/Cas9, pronuclear microinjection, isolation and culture/manipulation of pre- and post-implantation embryos, embryo transfer, embryo electroporation and roller bottle culture, chimera generation, generation and differentiation of mouse embryonic stem cells as well as induced pluripotent stem cells, isolation of mouse embryonic fibroblasts, vibratome and cryosectioning, in situ RNA hybridization, immunostaining, FACS sorting and analysis, vascular injections and casting, neural sterotaxic injections, skeletal preparation, organ explant culture and fluorescent imaging, including live time-lapse microscopy and tumors.

2015 Speakers:
Ben Arenkiel, Baylor College of Medicine
Mario Capecchi, University of Utah School of Medicine
Mikala Egeblad, Cold Spring Harbor Laboratory
James Glazier, Indiana University, Bloomington
Kat Hadjantonakis, Memorial Sloan Kettering Cancer Institute
Stacey Huppert, Cincinnati Children's Hospital Medical Center
Ophir Klein, University of California, San Francisco
Robin Lovell-Badge, MRC National Institute for Medical Research, UK
Scott Lowe, Memorial Sloan-Kettering Cancer Center
Ivan Maillard, University of Michigan
Joshua Sanes, Harvard University
Davor Solter, Institute of Medical Biology, Singapore
Xin Sun, University of Wisconsin, Madison
Patrick Tam, University of Sydney, Children's Medical Research Institute, Australia
David Threadgill, Texas A&M University
Paul Trainor, Stowers Institute for Medical Research
Harold Varmus, National Cancer Institute, NIH
Haoyi Wang, The Jackson Laboratory
Terry Yamaguchi, National Cancer Center/NIH

This course is supported with funds provided by the National Cancer Institute

Cost (including tuition, board and lodging): $4,705