Genome Engineering: CRISPR Frontiers
August 11 - 15, 2026

You must register for the meeting in order to submit abstracts.
After registering you will be sent a web link for abstract submission by email.
You may copy and paste your abstract from Word, Google Docs, or Notepad; abstracts are limited to ~2900 characters.

Program information: An electronic version of the program abstract book will be sent three working days prior to the first day of the meeting, and hard copies will be available for collection upon your arrival at Cold Spring Harbor Laboratory. First night and keynote speakers are informed of their session date and time, otherwise program information is only available upon release of the electronic version of the abstract book. The reason we do this is to try and maximize interactions by encouraging participants to stay for the duration of the meeting.

Please check your email for talk length, poster instructions, and how to have your poster printed at CSHL for collection upon arrival. 

Abstract Status

Presenting Author

Abstract Title

Talk/Poster

Achary, Vanpalli Mohan M

Development of low-rancidity, shelf-stable pearl millet flour via targeted genome editing

poster

Ahn, Chul Woo

Population-informed regulatory STRs at PITX2/PANCR nominate CRISPR-editable targets for atrial fibrillation functional genomics

poster

Ahouefa Adanho, Corynne Stephanie

Scale up CRISPR/Cas9 and base editing of human CD34+ HSPCs using peptide-mediated RNP delivery for translating to an ex vivo therapy for sickle cell anemia

poster

Anderson, Rachel A

Elucidating the genetic determinants of ABE editing using CRISPRi screens

talk

Aouida, Mustapha

Enhanced cellular uptake of compact Cas proteins—A comparative study of Cas12f and Cas9 in human cells

poster

Arceneaux, Aster

A compact, PAM-independent TasR gene-editor for single-vector exosomal-encapsulated-AAV delivery in vivo

poster

Aspiras, Ariel C

Adapting PASSIGE for targeted insertion of CFTR Super Exons into the CFTR genomic locus

poster

Bajpai, Ruchi

DNA barcoding measures diversity of genome edited cell pools and tracks modified alleles

poster

Barrera, Shirley

Enhancing CRISPR-mediated HIV-1 proviral targeting through optimized SaCas9 tracrRNA design

poster

Bauer, Jackson H

Base and prime editing to correct the most common non-deletional mutation in alpha thalassemia

poster

Blackwood, Stephanie

Developing a therapeutic in vivo prime editing strategy for MCAD deficiency

poster

Bondy-Denomy, Joseph

Diverse mechanisms of CRISPR-Cas evasion by bacteriophages

talk

Bonuzzi, Ilaria

In vivo homology-independent targeted integration mediated by all-in-one AAV delivery of a novel engineered TnpB genome editor

poster

Borah, Ashir A

PerturbAgent—An agentic framework for closed-loop discovery in perturb-seq

poster

Boros, Daniel

Exon-sized random mutagenesis of human chromosomes using mutagenic retron elements

talk

Bouillet, Antonin

New developments to CRISPOR for end-to-end design of genome editing experiments

poster

Brandao, Karina

Optimizing prime editing for precise genome engineering in cancer cell models

poster

Brooks, Alan

Targeted insertion of Factor VIII gene using a Type V CRISPR system results in dose dependent and durable Factor VIII expression in nonhuman primates

poster

Buchholz, Frank

Gene editing platforms for innovative health solutions

talk

Buffington, Jesse D

Engineering retron editors for robust mammalian gene editing

poster

Carbajal Contreras, Hector

Mitigating adverse CRISPR effects in functional cell-culture studies

poster

Carnevale, Julia

Virus-like particles enable targeted gene engineering and pooled CRISPR screening in primary human myeloid cells

talk

Chaudhry, Gautam

Next-generation modeling of complex genetics in vivo with multiplexed, inducible prime editing

poster

Chauhan, Vikash P

Engineered prime editors with minimal genomic errors

talk

Chen, Evelyn

In vivo base editing screens uncover genetic modifiers of CAR-T cell therapy response in pancreatic cancer

poster

Chen, Fei

Mapping regulators of tissue regeneration with scalable in vivo CRISPR screening

talk

Chen, Honglin

Target RNA abundance controls the collateral activity of RfxCas13d in human cells and zebrafish embryos

poster

Chen, Wei

De novo design of genome regulators

talk

Chi, Hongbo

Next-generation CRISPR screens enable causal systems biology in immuno-oncology

talk

Cialek, Charlotte

IDT custom guide RNAs and CRISPR off-target solutions—Early research to therapeutic development

poster

Conklin, Bruce

Leveraging human genetic variation to therapeutically target hundreds of genes with dominant and dispensable disease alleles

poster

Cordes, Stefan

Consilience—A single-cell multiomic framework for designing cis-regulatory perturbations of T-cell state

poster

Cowan, Quinn

Manipulating DNA repair pathways to alter excision and inversion profiles in post-mitotic cells

poster

Cristian, Ana

Efficient prime editing in vivo and in vitro using lipid nanoparticles

talk

Crosby, Kadin T

Determining the mismatch inhibition mechanism of Cas12a2 for selective cell killing

poster

Csoma, Balazs

Design principles and usage of alternative pegRNAs

poster

Damodaran, Arun

Systematic single-cell exon deletions map alternative splicing-driven gene regulatory programs

poster

Daneshpajouh, Amirhossein

A distribution-free certificate for the off-target miss-rate of clinical CRISPR guides and the limits of what can be certified

poster

Dhanjal, Soniya

Enabling CRISPR-based functional genomics through national research infrastructure—The SciLifeLab CRISPR Functional Genomics Unit

poster

Docter, Trevor

VIPR RNA-guided DNA recognition by noncontiguous geometric triplex formation

talk

Eid, Ayman

Multi-component optimization of SHARC CRISPR tools in plants

poster

Eitzinger, Simon

Molecular requirements of efficient DNA integration with evoCAST

talk

Erwood, Steven

Engineering of compact prime editors by sequence-function mapping

poster

Eshleman, James R

Can CRISPR-Cas9 genetically kill cancer?

poster

Fatma, Shirin

Structure of the Type I-F3 CAST holo integration complex reveals licensing mechanisms during RNA-guided DNA integration

talk

Fayer, Shawn

Editing stem cell genomes at scale to measure variant effects in diverse cell and genetic contexts

talk

Fuller, Mary Gracen A

Characterization of large serine recombinases (LSRs) for genome engineering in Lactobacillus gasseri

poster

Gál, Luca

Systematic evaluation of the effect of PBS length on editing outcome in prime editing

poster

Ganesan, Vijaydev

Development of a high-efficiency, multiplexed CRISPR-Cas9 toolkit in non-model oleaginous yeasts for enhanced xylitol production

poster

Gao, Alex

Beyond CRISPR—Phage protein sensing and host factor repurposing in bacterial immunity

talk

Gautier, Megan K

In vivo corrective editing of the liver improves brain network dynamics in a mouse model of phenylketonuria

poster

Gibson, Daytan

Base editing tiling screens with prime editing-based saturation mutagenesis as a strategy to map ligand-specific resistance

poster

Gladkov, Gregory T

A genome-wide CRISPR loss-of-function screen in primary human CD4+ T cells identifies novel host regulators of HIV-1 Envelope biogenesis and broadly neutralizing antibody (bNAb) binding

talk

Gordan, Raluca M

Intrinsic dCas9-DNA binding behaviors reveal mechanisms of ineffective and promiscuous guides in epigenome editing

talk

Gu, Bo

Programmable pathway profiles reveal signaling principles of TGF-β superfamily receptors

talk

Guffy, Sharon L

SHARC cytosine base editor optimization for agricultural applications

talk

Gupta, Nikhil

Scarless conditional guide RNA activation enables temporal, rapid and non-leaky CRISPR perturbation

poster

Gupta, Ria

Linking CRISPR perturbations to live-cell behavior, morphology, and paired transcriptomics in single cells

poster

Haas, Johannes

Direct repeat engineering enables robust Cas13d multiplexing for combinatorial RNA perturbation screens

poster

Hacker, Julia

Comparative efficacy of LNP-delivered base editing strategies in Rosa26 knock-in versus minimally humanized mouse models for multiple pathogenic variants of metabolic disease

poster

Hamilton, Jennifer R

Programmable in vivo engineering of T cells via cell-targeted CRISPR delivery and site-specific gene integration

talk

Han, Huajun

Perturb-seq of select Crohn’s disease candidate genes in human monocyte-derived macrophages defines cell states and fates

poster

Haupt, Amanda

Decoupling deaminase recruitment reveals a Cas association-dependent origin of off-target base editing

poster

Held, Friederike

Latency-resolved CRISPR perturbation of EBV+ B cells to dissect host-permissive states, EBV genomic variants, and EBV-host interactions in multiple sclerosis

poster

Hempstead, Andrew

Fostering scientific discovery and innovation through research tool sharing

poster

Herail, Theo

Development of a prime editing tool for the generation of protein diversity in eukaryotic cells

poster

Hernandez-Reyes, Jayvin

Safe Harbor-enabled high-throughput screening of gene editing tools in inherited retinal disease

poster

Hess, Gaelen

Scalable identification of function-modulating mutations using diversifying base editors

poster

Hirabayashi, Shigeki

Functional interpretation of GATA2 variants through GenoPrime-Perturb-seq

talk

Hong, Soonwoo

AI-guided CRISPR-based tuberculosis drug resistance detection

poster

Hsing, I-Ming

DNA guided CRISPR Cas12a—Reprogramming the activation pathway for programmable RNA targeting

poster

Hsiung, Chris

Higher-order combinatorial functional genomics

talk

Hu, Hannah

Genetically modifying patient-derived induced pluripotent stem cells to rescue autosomal dominant retinal disorders for autologous transplantation therapy

poster

Iohannes, Sessen D

A protein-responsive feedback circuit underlies transcriptional compensation in plant stem cells

talk

Jackson, Ryan N

Molecular basis of activating Cas12a2

poster

Jancola, Kyle

Identification of pathogenic DNA mismatch repair variants conferring increased cancer risk

poster

Jeon, Youngkyu

Combinatorial CRISPR screening uncovers genetic interaction networks underlying metabolic plasticity in cancer cells

poster

Jinek, Martin

Molecular mechanisms of RNA-guided transposons and recombinases

talk

Jo, Ilhyeong

Optimization of tomato gene promoters and regulatory elements for transgene expression and prime editing in Nicotiana benthamiana

poster

Joe, Joanna

Modification of Easi-CRISPR knock-In protocol to create an Atm inducible mouse model of ataxia telangiectasia

poster

Jowdy, Casey C

Enhancing T cell fitness through genome engineering using hfCas12Max delivered via mechanoporation

poster

Judge, Luke

Mutation-specific and mutation-agnostic inactivation of a dominant-negative disease allele ameliorates spinal motor neuropathy in vivo

poster

Jung, Hyuncheol

Virus-like particles enable targeted gene engineering and pooled CRISPR screening in primary human myeloid cells

poster

Justice, Brandon M

Unlocking extra-hepatic therapeutics with compact gene editors

poster

Kadirkamanathan, Renuka

Genome editing strategies for fragile DNA disorders

poster

Kahn, Elena

Correction of a recurrent pathogenic variant in methylmalonic acidemia using adenine base editing

poster

Kennedy, Jesse G

Don’t stop at red lights—CRISPR prime integrase for targeted knock-out and replacement in a novel traffic light assay

poster

Khan, Misbah

A bacteria-free CRISPR–HDR workflow for editing episomal KSHV genomes directly in mammalian cells

poster

Kimihira, Tetsutaro

Development of a novel transcriptional activation tool based on type I-E CRISPR-Cas

poster

Kinney, Kyle

A programmable UNA guide architecture improves CRISPR genome-editing specificity across genomic loci

poster

Kipp, David W

Quantification of early-disease biomarkers in a mouse model of IMPG2-mediated retinal degeneration

poster

Koblan, Luke W

Comprehensive lineage tracing maps the landscape of cell fate decisions in mouse embryogenesis

talk

Komor, Alexis C

Engineering and optimizing precision genome editing tools

talk

Kraus, Carolyn

Myo-miR restricted muscle genome editing in mice

poster

Ku, An-Chi

Engineered virus-like particles (eVLPs) as a versatile and safe delivery platform for advanced CRISPR genome editing

poster

Kulcsar, Peter

In vivo genome editing of MC4R prevents and reverses obesity in mice

poster

Lai, Zheng-Sheng

ITR-flanked single-stranded DNA is not the sole determinant of efficient CRISPR-mediated knock-in using AAV-delivered donor templates

poster

Larkin, Hailey

Efficient large-fragment CRISPR knock-in enables a fluorescent reporter cell line for adipogenic differentiation studies

poster

Lee, Wei Ting Chelsea

Enabling CRISPR-mediated homology-directed repair in somatic cells in vivo

poster

Li, Chaozheng

We combine our proprietary AI-powered gene editing platform, CasN, with machine learning-driven sgRNA and ssDNA design algorithms to deliver fast, cost-effective, and high-quality gene knock-in and knockout services at scale

poster

Li, Wei

pertTF—Context-aware AI modeling for genome-scale and cross-system perturbation prediction

poster

Li, Weilin

Investigating the roles of cell polarity protein RACGAP1 in regulating gemcitabine resistance in pancreatic cancer

poster

Liang, Xiquan

Vivofectamine delivery solutions—Lipid nanoparticles for in vivo gene editing in the liver

poster

Liu, Bin

Prime assembly with linear DNA donors enables large genomic insertions

talk

Liu, David R

Mutation-specific, mutation-agnostic, and disease-agnostic therapeutic genome editing

poster

Liu, Shawn

Mutation-agnostic silencing of pathological alleles by CRISPR-IMPRINT

poster

Liu, Yanjing

Combinatorial engineering identifies potent novel activators for scalable CRISPR activation

talk

Lobo, Tatiana

FAM50A synthetic lethality in solid tumors—A biomarker-driven therapeutic opportunity

poster

Loveless, Theresa B

Engineerable RNA Polymerase III promoters for gene editing and DNA recording

talk

Lu, Xiaona

Brain-wide non-viral genome editing for angelman syndrome using STEP-RNP delivery in non-human primates

talk

Lucas, Julie L

Decoupling CRISPR recognition and signal generation through binding based capture

poster

Lukarska, Maria

Activity and specificity trade-offs in adenine base editors

poster

MacVeigh-Fierro, Daniel T

Enhancing the purity and efficacy of click editing through chemically modified templates

poster

Manabayeva, Shuga

Development and optimization of CRISPR/Cas9 system elements for studying drought resistance mechanisms in potatoes

poster

Maxwell, Karen L.

Rewriting DNA repair for bacterial immunity—Widespread glycosylases target modified phage genomes

talk

McDiarmid, Troy A

Large-scale discovery of neural enhancers for cis-regulation therapies

poster

McVicker, Graham

Joint single-cell capture of Cas9 edits and transcriptomes reveals on- and off-target effects on gene expression

poster

Meganathan, Kesav

A quality control framework for CRISPR-engineered iPSCs in drug discovery

poster

Mercer, Heather

Experimentally informed model of CRISPR-CAS9 site affinity

poster

Miller, Blake

Inhaled delivery of gene editors to the distal lung via polymer 81

poster

Mller, Rasmus

Endogenous microRNA logic gates for spatiotemporal control of CRISPR/Cas systems

poster

Moffat, Jason

Integrative CRISPR approaches and annotation efforts for precision disease modeling

talk

Mondal, Indranil

Saturation prime editing coupled to a fluorescent miRNA-activity reporter resolves DICER1 variant pathogenicity at scale

poster

Moon, Tae Seok

CRISPR-mediated engineering of microbes and microbiota for real-world applications

poster

Mustafa, Yasmin M

Optimization of prime editing and eePASSIGE-mediated targeted transgene integration into safe harbor locus in sheep fetal fibroblast cells

poster

Myhrvold, Cameron

Intersegmental transfers drive target search in an RNA-targeting CRISPR system

talk

Nagatomo, Daiki

Optimization, enhancement, and modification of PAM preference in CRISPR-Cas3-mediated gene knock-in with long DNA replacement

poster

Nakamae, Kazuki

PtWAVE—Sanger trace deconvolution software for sensitive detection of large deletions at genome-editing target sites

poster

Napoliello, Renee

Guide RNA and Cas9 protein structure predict CRISPR outcomes across experimental contexts

poster

Nayfach, Stephen

Programming PAM specificity for mutation-specific base editor design

talk

Newcombe, Kevin L

The novel recombinase Brec1 recombines HIV-1 and acts on integrated provirus

poster

Nguyen, Long T

Engineering TIGR-TasR as a hypercompact PAMless RNA-guided genome editor for mammalian cells

poster

Noun, Tala

Genetic interactions delineate roles of chromatin remodeling complexes in EMT

poster

Orosco, Carlos

DNA-guided CRISPR-Cas12 for cellular RNA targeting and DNA-RNA editing

poster

Ortego, Michael S

Investigating the impact of excess gRNA and multiplexing on RNP efficiency

poster

Osgood, Nicola

Your New BESTIE—A high-throughput screening tool to interrogate base editing outcomes

poster

Pandala, Niharika

A scalable computational workflow for CHANCE-seq off-target profiling of CRISPR nucleases and base editors

poster

Patel, Nikhil

High-dimensional optical screens reveal organizing principles of biomolecular condensate assembly

talk

Patinios, Constantinos

Natural DarT toxin diversity expands Append Editing into a modular genome editing platform

poster

Peled, Eden

Base editing rescues survival and neurodevelopmental deficits in a mouse model of GRIN2D developmental epileptic encephalopathy

poster

Pereira, Arthur O

Engineering CRISPR-Cas3-mediated phage resistance in Xanthomonas citri

poster

Perrotta, Ramiro M

Engineered base editors with reduced bystander editing through directed evolution

talk

Peters, Samuel T

Hidden structural complexity of rAAV-mediated knock-in alleles revealed by LOCK-seq

poster

Pham, Vi

Correction of a recurrent pathogenic variant in multiple sulfatase deficiency using adenine base editing

poster

Pinello, Luca

From long reads to personal genomes—Computational tools for measuring genome editing outcomes and predicting off-target risk across diverse populations

talk

Policastro, Bob

Base editor genomic safety assessment: keeping pace with emerging FDA expectations

poster

Prieto Vivas, Julian

CoMuTER 2.0: A Cas3 based platform for confined, kilobase scale in vivo mutagenesis

talk

Qasim, Waseem

First-in-human trials of TALEN, CRISPR/Cas9 and base-edited CAR T cells

talk

Qi, Chen

BioPrinCRISPR—A biological principle-informed framework for discovery of novel CRISPR-Cas systems

poster

Quidwai, Tooba

A WDR35-dependent coat protein complex defines a vesicular trafficking route to cilia and a genome-edited platform for studying ciliopathies

poster

Raguram, Aditya

Genome-wide screening reveals producer-cell modifications that improve virus-like particle production and delivery potency

talk

Rakestraw, Noah

Programmable ligation-based genome editing with HUH endonucleases

poster

Ramirez, Josephine

Structural insights into the mechanism of telomerase-like DNA synthesis by an antiviral reverse transcriptase

talk

Ramsden, Dale A

Mechanisms for end-joining repair of chromosome breaks

talk

Ressnerova, Alzbeta

Modular, self-delivering CRISPR RNP platform for in vivo genome editing across diverse tissues

talk

Riching, Andrew

A bidirectional Dual-sgRNA system for uniform and efficient multi-gene CRISPR perturbation

poster

Rößler, Leon Marvin

Prediction of DNA donor efficiency for effective CRISPR editing

poster

Roth, Goldie V

Programmable surface receptor-mediated cell targeting with designer antibody-routed virus-like particles

talk

Ruis, Brian L

The Shu complex regulates oligonucleotide-mediated, Cas9 nickase-initiated gene editing in human cells

poster

Saitova, Elizaveta

Machine learning-guided site-specific recombinase engineering for correction of DMD duplications

poster

Sanchez, Henry

High-throughput prime tagging of endogenous proteins for pooled imaging of protein localization

talk

Schneider, Stefan

Megabase genome engineering toward a minimal CHO cell chassis for the production of biopharmaceuticals

poster

Shi, Honglue

Stepwise DNA unwinding gates TnpB genome-editing activity

talk

Shi, Junwei

Small-molecule controlled base editing for high-efficiency in vivo cancer functional genomics

poster

Shin, Jee Won Jennifer

Spatial multiomic CRISPRi approach to map lipid metabolism gene function in MASLD

poster

Shui, Bing

Next-generation modeling of complex genetics in vivo with multiplexed, inducible prime editing

talk

Skopintsev, Petr

Structure and evolution-guided design of minimal RNA-guided nucleases

poster

Smith, C. I. Edvard

Genetic and functional correction of X-linked agammaglobulinemia (XLA) by CRISPR adenine base editors

poster

Srikanth, Smriti

Optimized CRISPR interference guide design considerations and performance in perturb-seq data

poster

Starita, Lea

Saturation genome editing at scale—From variant effect maps to clinical evidence

talk

Sturgeon, Morgan

Accelerated population-aware gRNA candidate selection using high-throughput nomination methods

poster

Sugiyama, Ken

Development of a novel inducible prime editing system using split reverse transcriptases

poster

Sun, Mandy X

A modular virus-like particle platform for transient in vivo delivery of genome and epigenome editors

talk

Talas, Andras

In vivo rescue of a urea cycle disorder via RNA-LNP-mediated prime editing

talk

Tao, Allen

AI-guided redesign of laboratory-evolved reverse transcriptases enhances prime editing

talk

Theva Das, Kumitaa

Silver nanoparticle-multiplex CRISPR/Cas9 for HIV gene therapy—Blood-brain barrier protection and in vivo safety evaluation

poster

Thompson, Jared

RNA-triggered cell elimination with CRISPR-Cas12a2

talk

Tran, Paul M

Rapid identification of viruses and ligands (RIVAL)—A miniaturized, multiplexed CRISPR/Cas12a platform for accelerated viral receptor discovery

talk

Tsujimoto, Eisuke

Structural mechanism of DNA recognition by the CCA domain in an IS110 bridge recombinase

poster

Tussipkan, Dilnur

Molecular mechanisms underlying defense responses of potato (Solanum tuberosum L.) and employing CRISPR/Cas technology for the improvement of potato

poster

Vedova, Shane A

CRISPR screening to decode mechanisms of T cell persistence

poster

Villiger, Elina

Prime editing of a pathogenic Scn1a allele ameliorates seizure phenotypes in a GEFS+ mouse model

poster

Walker, Sadie

Bridging donors and NHEJ inhibitors enhance large deletions after concurrent double-strand breaks

poster

Wamsley, Jake

Efficient HDR-mediated genome editing in primary human T cells using mbDNA™ donor templates and high-fidelity eSpOT-ON nuclease

poster

Wang, Siyuan (Steven)

Image-based spatial transcriptomics of native and guide RNAs

talk

Wang, Xiao

In vivo correction of pseudoxanthoma elasticum via base editing

talk

Wang, Zhaojun

Orthologs of trans DNA-cleaving nucleases for RNA-triggered cell elimination

poster

Wasko, Kevin M

Reprogramming T cells for intercellular delivery of genome editors

poster

Watkins, Leslie

A pervasive quantification artifact inflates RNA knockdown by RNA-targeting CRISPR

talk

Wilkinson, Max

Discovering new RNA-based biology in prokaryotes and their viruses

talk

Wu, Xiaolong

DNA-guided CRISPR–Cas12a for RNA detection and intracellular RNA knockdown

poster

Zeng, Jingkun

CRISPR-programmed chromatin shredding for disease treatment

talk