Genome Engineering: The CRISPR/Cas Revolution
August 22 - 25, 2018

You must register for the meeting in order to submit abstracts.
After registering you will be sent a web link for abstract submission by email.

Program information: An electronic version of the program abstract book will be sent three working days prior to the first day of the meeting, and hard copies will be available for collection upon your arrival at Cold Spring Harbor Laboratory. First night and keynote speakers are informed of their session date and time, otherwise program information is only available upon release of the electronic version of the abstract book. The reason we do this is to try and maximize interactions by encouraging participants to stay for the duration of the meeting.

Please check your email for talk length, poster instructions, and how to have your poster printed at CSHL for collection upon arrival. 

Abstract Status

Presenting Author

Abstract Title

Talk/Poster

Agudelo, Daniel S

Versatile in vivo genome editing with the prototypical Streptococcus thermophiles CRISPR1-Cas9 system

poster

Allen, Felicity

Systematic analysis of CRISPR/Cas9-generated mutational profiles using synthetic self-targeting gRNA constructs

poster

Allen, Felicity

JACKS—Joint Analysis of CRISPR/Cas9 Knockout Screens

poster

Aman, Rashid

RNA virus interference via CRISPR/Cas13a system in plants

poster

Aregger, Michael

Global mapping of metabolic genetic interaction networks using CRISPR-Cas screens

poster

Ata, Hirotaka

Double-stranded break repair made predictable: single mutant allele predominates over any other by rational target selection in vivo

poster

Awah, Chidiebere

Genome-wide CRISPR Cas9a knockout screen identifies biomarkers for predicting glioblastoma susceptibility to TOP2 poisons

poster

Bachle, Susanna

CRISPR plasmid technology—Data and tool evolution trends from Addgene

poster

Bandyopadhyay, Anindya

Enhancing plant breeding and product development by CRISPR systems

poster

Bassik, Michael

Discovery of general and context-specific regulators of phagocytosis using magnetic genome-wide CRISPR screens

talk

Bello, Erica

A CRISPR-scan assay for the functional characterisation of recurrent mutations in cancer

poster

Beying, Natalja

SSB-induced homologous recombination in Arabidopsis thaliana

poster

Biswas, Kajal

High-throughput BRCA2 variant analysis using mouse ES cell-based assay

poster

Bonafont Arago, Jose

Novel efficient CRISPR/Cas9-based gene editing strategies for recessive dystrophic epidermolysis bullosa

poster

Boyle, Evan A

Resolving core genes for cancer growth from high-throughput genetic screen data: applications from a co-functional gene network

talk

Brokowski, Carolyn E

The ethical conduct of clinical trials involving novel genomic engineering tools

poster

Bruhn, Laurakay

Improving CRISPR-Cas9 specificity with chemical modifications in single-guide RNAs

poster

Chang, Yong S

Development of NTLA-1001—First-in-class, LNP-CRISPR/Cas9 mediated genome editing therapeutic for the treatment of ATTR

talk

Chen, Honglin

Somatic genome editing in murine lung via AAV delivery of CRISPR

poster

Chen, Jia

Base editing with a Cpf1–cytidine deaminase fusion

poster

Chen, Sidi

Advances in multiplexed gene editing and in vivo genetic screening

poster

Chen, Wei

Predicting the end-joining patterns of CRISPR/Cas9-mediated double stranded breaks from thousands of programmed sequences

poster

Chokshi, Chirayu

Discovery and validation of genes essential for survival of recurrent Glioblastoma brain tumor initiating cells

poster

Chong, Shan

Extracellular receptor-ligand interaction screening using CRISPR activation

poster

Choudhary, Amit

Synthetic activators, inhibitors, and degraders of CRISPR-associated nucleases

talk

Ciccia, Alberto

Modulating DNA repair to enhance precise genome editing

talk

Cogan, James Z

Reprogramming the epigenome with next generation dCas9 fusions

poster

Conant, David F

Methods for predicting repair genotype of CRISPR-Cas9 mediated edits

poster

Concordet, Jean-Paul

Impact of DNA double strand break repair pathways on genome editing

poster

Conklin, Bruce

Silent repair, a path to scarless genome surgery

talk

Connelly, Jon P

Whole genome CRISPR-based KO screen to identify modulators of single strand templated repair

poster

Corda, Gabriele

A knock-out genome-wide screen identifies novel potential therapeutic targets for the treatment of Friedreich’s ataxia

poster

Cox, Kurt

High-throughput platforms to identify inhibitors of CRISPR-Cas9

poster

Daboussi, Fayza

One-step generation of multiple gene knock-outs in diatoms by DNA-free genome editing

talk

Dabrowska, Magdalena

qEva-CRISPR—A method for quantitative evaluation of CRISPR/Cas-mediated genome editing in target and off-target sites

poster

D'Angelo, Michael E

A new unbiased method for finding CRISPR off-target cut sites in live cells.

poster

Danner, Eric W

Homology independent replacement of sequence

poster

Dao, Thi Mai Lan

CRISPR-based approach for HBB gene correction in human CD34+ Hematopoietic stem cells

poster

Davidson, Alan R

Crunching CRISPR—The many mechanisms of CRISPR-Cas inhibition by anti-CRISPRs

talk

de Onate, Lorena

Liver tissue specific delivery and editing by receptor-mediated uptake of CRISPR-Cas9

poster

Dede, Merve

Identifying novel genomic vulnerabilities in human cancer cells through characterization of gene essentiality profiles

poster

Dersh, Devin

Genome-wide CRISPR/Cas9 knockout screens identify regulators of MHC Class I in diffuse large B-cell lymphoma

poster

Desplan, Claude

Evolution of sensory perception

talk

Dow, Lukas

Optimized base editors enable efficient editing in cells, organoids, and mice

poster

Duringer, Alexis

Identification and characterization of an S phase-specific degron with potential application to CRISPR-Cas9-based genome editing

poster

Edraki, Alireza

A compact, high-accuracy Cas9 with a dinucleotide PAM for in vivo genome editing

poster

Enciso-Rodriguez, Felix E

Overcoming self-incompatibility in diploid potato using genome editing

poster

Faure, Guilhem

Mini CRISPR array, a weapon in phage wars

talk

Fellmann, Christof

CRISPR-Cas9 circular permutants as programmable scaffolds for genome modification

poster

Finnigan, Gregory C

Tuning CRISPR/Cas gene drives in budding yeast—Design, complexity, and inhibition

poster

Fraser, Hunter

Functional genetic variants revealed by massively parallel precise genome editing

talk

Frock, Richard L

Ku70 suppresses alternative end-joining in G1-arrested progenitor B cells

poster

Fuchs, Ryan

In vitro characterization of Cas12a (Cpf1) endonucleases as tools for molecular biology

poster

Gallagher, Danielle N

Single strand template repair—Characterization of a novel RAD51-independent pathway

poster

Gangopadhyay, Soumyashree A

High-resolution spatiotemporal control of CRISPR-Cas9 using photocaged small molecules

poster

Gasior, Stephen

Targeted gene activation with a type I CRISPR-Cas system in plants

poster

Ghanta, Krishna S

5′ modifications improve potency, efficacy and tolerability of DNA donors for precision genome editing

poster

Gonatopoulos-Pournatzis, Thomas

Development and application of novel CRISPR-based screens for investigating the regulatory and functional complexity of alternative splicing

poster

Grevet, Jeremy

Domain­-focused CRISPR­-Cas9 screen identifies HRI kinase as a regulator of fetal hemoglobin in adult human erythroid cells

talk

Gu, Bin

Efficient generation of targeted large insertions by microinjection into two-cell stage mouse embryos

talk

Guo, Haotian Q

All-or-none responses of Csy4 to the substrate's flanking sequences and machine learning of the selection rules

poster

Gurskaya, Nadya

Modelling epidermolysis bullosa simplex with CRISPR/Cas/9 system

poster

Gurumurthy, Channabasavaiah B

In vivo germ-line genome editing in rodents

talk

Haapaniemi, Emma M

CRISPR-Cas9 induces a p53-mediated DNA damage response

talk

Haining, Nicholas

In vivo genetic screens to discover targets for cancer immunotherapy

talk

Haurwitz, Rachel

Precision genome editing—Identification of highly efficient and specific guides for CRISPR-Cas9 gene editing at human T cell loci

talk

He, Jiankui

CRISPR Off-target analysis in human embryo

poster

Heffel, Matthew G

Computational modeling of tunable CRISPR gene drive population dynamics

poster

Hess, Gaelen

Targeted diversification via dCas9 mediated base editing

poster

Heyza, Joshua R

Mechanisms of resistance to interstrand crosslinks with ERCC1 deficiency

poster

Hidalgo Cantabrana, Claudio

Repurposing the endogenous CRISPR-Cas I-E system for genome editing in Lactobacillus crispatus

poster

Holden, Kevin

Inference of CRISPR Edits (ICE)—A free, online analysis tool for multiplex CRISPR edits

poster

Huang, Jeffrey Y

Developing CRISPR-Cas9 knock-in models of rare pediatric disorders to better characterize pathogenesis and evaluate novel therapeutics

poster

Hulton, Chris

A CRISPR-Cas9 system for direct genome editing in lung cancer patient-derived xenografts

poster

Ivanova, Marianna

Phenotype and genotype characterization of Usher syndrome in Russian cohort

poster

Iyer, Shruti V

CATCH me if you can—Characterization of BRCA1 structural variation using HLS-CATCH system and nanopore sequencing

poster

Jacobi, Ashley

A comprehensive study comparing on- and off-target levels of the most common forms of CRISPR/Cas9 guide RNAs

poster

Jacobs, Thomas B

High-throughput functional genomics in plants with pooled, combinatorial CRISPR screens

poster

Jensen, Emil D

Directed evolution with Cas9 and error-prone donor-coupled gRNA delivery

poster

Jensen, Michael K

Toolkits for multiplex CRISPR/Cas-mediated genome engineering, transcriptional reprogramming, and directed evolution in yeast

poster

Jiang, Tingting

In-vivo adenine base editing rescues disease phenotype in an adult mouse model of tyrosinemia

poster

Jinek, Martin

Structural insights into the mechanism of DNA cleavage by CRISPR-Cas12a/Cpf1

talk

Johnston, Robert K

Rapid electrochemical detection and quantification of Cas9 protein

poster

Jones, Stephen K

High-throughput profiling of DNA binding by engineered high-fidelity Cas9 variants

poster

Joung, J. Keith

Enhanced CRISPR-Cas12a variants with increased targeting ranges and activities for gene, epigenetic, and base editing

talk

Karlseder, Jan

Regulation of DNA repair pathway choice in S/G2 by the NHEJ inhibitor CYREN

talk

Kato, Tomoko

Genome editing via Cas9 RNP improves the HDR/NHEJ ratio

poster

Kelliher, Timothy

Genome editing in diverse crops and varieties via haploid induction

poster

Keough, Kathleen

ExcisionFinder—A software tool for personalized and allele-specific sgRNA design via incorporation of genetic variants

poster

Kleinstiver, Ben P

Enhanced activity and targeting range CRISPR-Cas12a variants for genome, epigenome, and base editing

poster

Kulcsar, Peter

Ranking between high fidelity SpCas9 nucleases

poster

Kunii, Atsushi

Three-component repurposed technology for enhanced expression (TREE)—Highly accumulable transcriptional activators via branched tag arrays

poster

Lazzarotto, Cicera R

CHANGE-seq—A high-throughput method for defining CRISPR-Cas9 genome-wide activity in vitro

poster

Lee, Hye Young

Nanoparticle delivery of CRISPR into the brain rescues a mouse model of fragile X syndrome from exaggerated repetitive behaviors

talk

Leger, Brittany S

A synthetic gene drive with small-molecule contingency

poster

Lenoir, Walter F

Visualization tools of genetic interactions interpreted through genetic fitness screens

poster

Li, Wei

Identification of essential sites in the CTCF and FOXA1 cistromes using CRISPR screens

talk

Lin, Shu-Wha

Use of Quick Macro to increase efficiency when designing sgRNA for Cas9

poster

Lippman, Zachary

Gene editing in plants is here.  Now what?

talk

Liu, David R

Base editing—Chemistry on a target nucleotide in the genome of living cells

talk

Lu, Baisong

Delivering Cas9 mRNA by lentivirus-like particles for transient expression and efficient gene editing

talk

Lu, Quinn

Strategies for controlling off-target effects and biological variations in CRISPR/Cas9 genome editing experiments

poster

Luttgeharm, Kyle D

Automating CRISPR mutation detection and zygosity determination

poster

Ma, Tao

Genetic suppression of the kinase activity for mRNA translational factor eEF2 alleviates cognitive and synaptic impairments in a mouse model of Alzheimer’s disease

poster

Machado, Hidevaldo B

Gene knockout or gene activation screening with arrayed synthetic CRISPR libraries

poster

Maguin, Pascal

S. pyogenes type II-A CRISPR-cas system provides limited protection against Kayvirus phages

poster

Mair, Barbara

Mapping genetic dependencies in human embryonic stem cells by genome-wise CRISPR screening

poster

Makarova, Kira

Functional prediction and evolution of Class 2 CRISPR-Cas systems

talk

Marson, Alexander

Reprogramming human T cell circuitry with CRISPR

talk

Martyn, Gabriella E

Naturally occurring mutations disrupt binding of fetal globin repressor to elevate fetal hemoglobin

talk

McCaffrey, Anton

CleanCap allows diverse 5’ cap modifications—The RNA epitranscriptome

poster

McDonough, Justin A

Improving homology-directed repair efficiency in human stem cells

talk

McNeill, Matthew S

The novel Alt-R® CRISPR HDR Design Tool for ssDNA homology-directed repair template design

poster

Mir, Aamir

Heavily and fully modified RNAs guide efficient Cas9-mediated genome editing

poster

Miyaoka, Yuichiro

Modified interactions between Cas9/Cas9 D10A nickase and genomic DNA enhances HDR

poster

Molla, Kutubuddin A

Multiplex CRISPR-mediated base editing in rice

poster

Morell, Montserrat

Screening successful homologous recombination events using a fluorescence-based method

poster

Morgens, David W

CRISPRi interaction map and targeted mutagenesis identify first-in-class mechanism of antiviral, retrograde trafficking inhibitors

poster

Nakade, Shota

Biased genome editing using the LoAD (local accumulation of DSB repair molecules) system

poster

Nelson, Christopher E

Long-term evaluation of genome editing outcomes for Duchenne muscular dystrophy

poster

Ohtsuka, Masato

Development of a reporter mouse model suitable for evaluation of in vivo genome editing efficiency

poster

Olson, Eric

Correction of Duchenne muscular dystrophy by genome editing

talk

Pellegrino, Enrica

Engineering a drug-inducible allelic series in human stem cells

poster

Perez-Bermejo, Juan A

Interaction proteomics and genome engineering unravel how BAG3 gene variants cause heart disease

poster

Peters, Samuel T

Optimizing genome editing rates with RNP delivery of CRISPR-Cas9 reagents

poster

Petris, Gianluca

AsCpf1 genome editing rescues cystic fibrosis splicing mutation 3272-26A>G in patient organoids

poster

Pickar-Oliver, Adrian

Targeted gene activation with type I CRISPR-Cas systems in mammalian cells

talk

Potter, Jason

Improved TALENS for editing in PAM deserts

poster

Preece, Roland

CRISPR-CAR lentiviral vector coupling transgene expression with targeted scission for the engineering of universal T cells

poster

Rees, Holly A

Characterizing and improving the specificity and applicability of base editing

poster

Rettig, Garrett R

A complete strategy for characterizing on- and off-target CRISPR/Cas9 editing events via target enrichment and high-resolution NGS analysis

poster

Riesenberg, Stephan

Efficient multiplexed precise genome editing of human pluripotent stem cells by modified DNA-dependent protein kinase catalytic subunit

poster

Romanienko, Peter J

Fine tuning of electroporation of CRISPR reagents into mouse embryos

poster

Rousseau, Beth A

Programmable RNA recognition and cleavage by N. meningitidis CRISPR-Cas9

talk

Rowe, Matthew H

Streamlined production, application, and analysis of pooled genome-wide sgRNA lentiviral libraries

poster

Roy, Kevin R

MAGESTIC—A platform for multiplexed CRISPR-HDR variant engineering and tracking at the genome-scale

poster

Russell, Alasdair

Allele-specific curative editing of patient-derived iPSCs

poster

Sadelain, Michel

T cell and stem cell editing for cancer immunotherapy

talk

Saunders, Thom

CRISPR/Cas9 genome editing pipeline for mice and rats

poster

Savage, David

Directed evolution of smaller CRISPR-Cas proteins using MISER

talk

Schimmel, Joost

Exploiting DNA repair knowledge in genome editing approaches

poster

Schwank, Gerald

Correction of autosomal recessive disorders via CRISPR-associated base editors in adult animals

poster

Seth, Meetu

Rapid and specific purification of Cas9-RNP from mammalian cells and tissues

poster

Sheltzer, Jason

CRISPR/Cas9 mutagenesis invalidates multiple genetic targets undergoing clinical trials in cancer

poster

Shen, Max W

Predictable and precise template-free editing of pathogenic mutations by CRISPR-Cas9 nuclease

poster

Shih, Justin W

Phylogenetic and evolutionary analysis of DNA repair gene families in plants

poster

Stark, Jeremy

Mechanisms of intrachromosomal deletion rearrangements, and other chromosomal break repair outcomes

talk

Stonebloom, Solomon

High-throughput genome engineering for industrial strain optimization

poster

Symington, Lorraine

Sequence context influences mutagenic repair of CRISPR/Cas9-induced double-strand breaks

talk

Talas, Andras

Sequence specificity of Cas9 variants

poster

Temesgen, Ayantu M

Employing the CRISPR-Cas9 technology to develop robust and high throughout degradation assays

poster

Tran, Mai H

Cpf1 proteins excise CRISPR RNAs from mRNA transcripts in mammalian cells

poster

Tran, Ngoc Tung

The efficient CRISPR/Cas9-based gene editing of FANCG locus in human hematopoietic stem cells: a potential gene therapy Fanconi anemia

poster

Trinh, Cong T

CASPER—A platform for developing effective CRISPR-Cas targets

poster

Tsai, Yi-Ting

CRISPR-based genome surgery for the treatment of autosomal dominant retinitis pigmentosa

poster

Tsang, Stephen H

CRISPR-based genome surgery for the treatment of autosomal dominant retinitis pigmentosa

poster

Turk, Rolf

Improved genome editing efficiency in CD34+ cells using single-stranded oligonucleotides

poster

Tycko, Josh

High-resolution CRISPR-Cas9 screen identifies constituent functional elements within enhancers and loop anchors

talk

Vakulskas, Christopher

Improved CRISPR-SpCas9 and -AsCas12a proteins mitigate off-target risk and improve on-target performance and reliability

poster

Varble, Andrew

Recombination between phages and CRISPR-cas loci disseminates immunity during infection

talk

Villiger, Lukas T

Treatment of a metabolic liver disease by in vivo genome base editing in adult mice

poster

Voytas, Daniel F

Genome editing and plant agriculture

talk

Walia, Rupali

Designing innovative cell culture models to study chronic wasting disease by precise gene replacement using the CRISPR toolbox

poster

Walter, David

CRISPR-Cas9—A versatile tool for identifying and validating synthetic lethal interactions

poster

Walter, Marius

Gene drive in herpesviruses

talk

Wang, Alan S

Identification of proteins at genomic loci

poster

Wang, Chia-Lin

CRISPR/Cas9 based development of RNAi rat models for drug discovery

poster

Wefers, Benedikt

Optimizing CRISPR/Cas9-mediated gene targeting in mice for disease modelling

poster

Wheatley, Matt

CRISPR/Cas9-enabled multiplex genome editing in Magnaporthe oryzae

poster

Wienert, Beeke

Discovery of in situ CRISPR-Cas off-targets by chromatin-immunoprecipitation of DNA repair factors

poster

Wilde, Jonathan J

Efficient zygotic genome editing via RAD51-enhanced interhomolog repair

talk

Will, Anja J

Enhancer composition and dosage control developmental gene expression.

poster

Woltjen, Knut

Precise human disease allele creation and correction through microhomology-mediated end joining

talk

Wong, Tatianna Wai Ying

Utilization of CRISPR/Cas9-based gene editing to restore the open reading frame in a novel DMD mouse model

poster

Wood, Andrew

How chromatin modifications influence CRISPR-Cas9 mutation frequency and the outcome of mutagenic DNA repair

poster

Worley, Jeremy

Identifying the molecular determinants of poor outcome in colon adenocarcinoma using VIPER and pooled single-cell CRISPR screening

poster

Wyman, Stacia K

Indel-based barcoding to track cellular engraftment of CRISPR-edited primary cells

poster

Yang, Li

Genome editing pinpoints single bases

poster

Yeo, Gene W

RNA modulation by CRISPR/Cas proteins

talk

Yoon, Mijung

Targeted base editing via RNA-guided cytidine deaminases in Xenopus laevis embryos

poster

Yourik, Paul

Kinetic comparison of Cas9 homologs

poster

Zhang, Hanrui

Genome-wide CRISPR screen in primary macrophages for discovery of novel regulators of macrophage efferocytosis

poster

Zhang, Jenny

Discovery of an anti-CRISPR protein that unlocks novel anti-CRISPR loci in diverse bacteria

talk

Zhang, Ning

Generation and characterization of tomato lines with CRISPR/Cas9-mediated mutations in candidate immunity-associated genes

poster