Genome Engineering: The CRISPR-Cas Revolution                    
July 21 - 23, 2017

You must register for the meeting in order to submit abstracts.
After registering you will be sent a web link for abstract submission by email.

Program information: An electronic version of the program abstract book will be sent three working days prior to the first day of the meeting, and hard copies will be available for collection upon your arrival at Cold Spring Harbor Laboratory. First night and keynote speakers are informed of their session date and time, otherwise program information is only available upon release of the electronic version of the abstract book. The reason we do this is to try and maximize interactions by encouraging participants to stay for the duration of the meeting.

Please check your email for talk length, poster instructions, and how to have your poster printed at CSHL for collection upon arrival. 

Abstract Status

Presenting Author

Abstract Title

Talk/Poster

Adamson, Brittany

Perturb-seq—A multiplexed single-cell CRISPR screening platform

talk

Agudelo, Daniel S

Marker-free co-selection for CRISPR-driven genome editing in human cells

poster

Alcott, Callison E

A druggable genome screen identifies treatment targets for Rett and MECP2 duplication syndromes

poster

Antony, Jisha

Role of cohesin mutations in myeloid leukemia

poster

Aregger, Michael

Global mapping of metabolic genetic interaction networks using CRISPR-Cas9 screens—A roadmap for therapeutic discovery

poster

Bae, JiHyun

Multiple sgRNAs with overlapping sequences enhance CRISPR/Cas9-mediated knock-in efficiency

poster

Baeumler, Toni A

Engineering synthetic signalling pathways with programmable dCas9-based chimeric receptors

poster

Baker, Candice N

Efficiently creating and validating mouse models using CRISPR/Cas9

poster

Bandyopadhyay, Anindya

Successful application of CRISPR-CPF1 in rice

poster

Bhowmik, Pankaj K

The CRISPR/Cas system for haploid plant cells—Challenges and progress of wheat genome editing using microspores

talk

Bier, Ethan

The many flavors of active genetics

talk

Bikou, Maria

Functional analysis of PIEZO1 using genome edited hiPSC-derived cardiomyocytes as a model system

poster

Billmann, Maximilian

Genome-wide identification of genetic interactions in human cells using CRISPR/Cas9

poster

Bolukbasi, Mehmet F

Cas9-Cas9 fusions provide a versatile genome editing platform

poster

Bondy-Denomy, Joseph

Inhibition of CRISPR-Cas9 with bacteriophage proteins

talk

Brito, Luciano A

Lack of E-cadherin during zebrafish craniofacial development and implications for orofacial cleft etiology

poster

Brokowski, Carolyn E

Scientific, ethical, & legal considerations in reviewing CRISPR the disruptor

poster

Brunet, Anne

The African killifish—A new vertebrate model to study aging and age-related diseases

talk

Buerckstuemmer, Tilmann

A scalable approach towards conditional gene inactivation based on artificial introns

poster

Burt, Austin

Modified mosquitoes for malaria control—Current status, requirements for success, and a step-wise approach for getting there

talk

Carette, Jan

Genetic knockout screens to study virus host interactions

talk

Casini, Antonio

Directed evolution of highly specific SpCas9 variants

poster

Champer, Jackson

CRISPR gene drives with reduced resistance potential

talk

Chaverra Rodriguez, Duverney D

Targeted delivery of CRISPR/Cas9 into the adult mosquito germline

poster

Chen, Fuqiang

Chromatin interacting protein domains improve gene editing efficiency of class 2 CRISPR-Cas effector endonucleases on endogenous targets in the human genome

poster

Chen, Jia

An enhanced base editing system

poster

Cheng, Albert W

Casilio—A modular platform for combinatorial (epi)genome editing and chromatin imaging

talk

Choudhary, Amit

Small molecule inhibitors and activators of CRISPR-Cas9

talk

Ciccia, Alberto

CRISPR-mediated base editing enables efficient disruption of eukaryotic genes through induction of STOP codons (iSTOP)

talk

Conklin, Bruce

Genome engineering human iPS cells to model and treat disease

talk

Cook, Peter J

Rapid ex-vivo and in-vivo modeling of brain cancer-associated genomic rearrangements

talk

Corbett, Sybilla

The EMBL-EBI CRISPR archive

poster

Cotta-Ramusino, Cecilia

Characterization of genomic rearrangements in response to CRISPR/Cas9-induced double-strand breaks

talk

Crawford, Emily D

Using targeted nucleases to enhance discovery and detection by deep sequencing

poster

Cuomo, Emanuela

A predictive approach to ‘On-Target’ drug resistance in oncogenic cellular models

poster

Dalal, Sorab

Generation of transgenic mice using sperm mediated gene transfer.

poster

Daley, Timothy P

Mixture models for estimating effect sizes in genome-wide CRISPRa/i screens

poster

D'Angelo, Michael E

CRISPR/Cas9 genome edited models of STAT3 activated lysosome mediated cell death in mammary epithelial cells

poster

Danner, Eric W

Knock-in strategies for gene therapy—Utilizing the non-homologous end joining repair pathway

poster

Das, Sunny

Targeting the macrosatellite repeat D4Z4 to develop therapeutic tools for Facioscapulohumeral muscular dystrophy

poster

Datlinger, Paul

CROP-seq—Pooled CRISPR screening with single-cell transcriptome readout

talk

Dorkin, Joseph R

Enhanced in vivo gene editing efficiency with single dose administration of CRISPR-Cas9 components formulated in proprietary lipid nanoparticles

talk

Dowdy, Christopher R

C57BL/6NCrl mouse models generated by CRISPR/Cas9-mediated gene-editing

poster

Doyle, Riley O

Evaluation of predictors of guide RNA performance across cell lines and species

poster

Enciso, Felix E

Overcoming self-incompatibility in diploid potato using genome editing

poster

Fan, Meng-Ni

Generation and application of MHC class I null immunodeficient mice with bleeding disorder

poster

Fan, Yong

Engineering TALENs specific targeted to mutant mitochondrial genomes leaving nuclear genome untouched

poster

Feng, Weiran

BRCA2 suppresses replication stress-induced mitotic and G1 abnormalities through homologous recombination

talk

Ferry, Quentin RV.

Rational design of inducible CRISPR guide RNAs for de novo assembly of transcriptional programs

poster

Gallagher, Danielle N

CRISPR/Cas9 genome editing—Characterization of a Rad51-indepdendent DNA repair pathway

poster

Gao, Huirong

Progress on applications of CRISPR-Cas enabled advanced breeding technology in trait development in maize

talk

Garcia Lopez, Jesus

Modeling high risk neuroblastoma through CRISPR/CAS9 genome editing

poster

Garyfallos, Dimitrios

Functional genomic studies of myeloid leukaemias using in vitro and in vivo CRISPR/Cas9 forward genetics screens

poster

Gauttam, Rahul

A dual-inducible CRISPR Interference system for the redirection of metabolic fluxes in Corynebacterium glutamicum

poster

Georgiadis, Christos

Homogenous T cell engineering using novel CRISPR-CAR coupled lentiviral vector platform

talk

Gersbach, Charles

Genome and epigenome editing for gene therapy and cell programming

talk

Gibb, Bryan

Protein dynamics of human RPA and RAD51 on ssDNA during assembly and disassembly of the RAD51 filament

poster

Gilbert, Luke

Towards a genetic interaction map of the human cell

talk

Gisler, Santiago

Regulatory elements associate with frequency of CRISPR-Cas9 induced insertions, but not deletions, in mouse embryonic stem cells

poster

Guay, David

Peptide technology to deliver CRISPR ribonucleoprotein in therapeutic cells

talk

Gurumurthy, Channabasavaiah B

CRISPR solution for creating Cre-LoxP genetics resources

poster

Haber, James

Rad51-independent single-strand template repair of site-specific double-stand breaks

talk

Haeussler, Maximilian

CRISPOR.org supports the whole process of genome editing

poster

He, Jiankui

Evaluating the safety of germline genome editing in human, monkey and mouse embryos

talk

Heidenreich, Matthias

Multiplex gene editing by CRISPR-Cas and Cpf1 in the mouse brain

talk

Hellebaut, Lana

Gene targeted insertion of the AP-MS tag in plant cells using CRISPR/Cas9

poster

Holden, Kevin

Use of synthetic sgRNAs for highly efficient CRISPR editing in various cell types

poster

Hsu, Jonathan Y

CRISPR-SURF—An integrative and streamlined pipeline for analysis of CRISPR-based non-coding tiling screens

talk

Huangfu, Danwei

Investigating human development through genome editing in pluripotent stem cells

talk

Humphryes-Kirilov, Neil A

Thera-Seq—A high-throughput deep sequencing approach for preclinical CRISPR off-target assessment

poster

Hynes, Richard

National Academies report on Human Genome Editing—Conclusions, questions and follow-up

talk

Jacobi, Ashley

Optimized design, modification, and delivery of the Acidaminococcus sp. Cpf1-gRNA ribonucleoprotein complex

poster

Jaenisch, Rudolf

Epigenetics, stem cells and disease research

talk

Jakimo, Noah

Chimeric CRISPR guides enhance Cas9 target specificity

poster

Jang, Yoo Jin

High efficient genetically modified mice generation with Campylobacter jejuni Cas9

poster

Jost, Marco

Combined CRISPRi/a-based chemical genetic screens reveal that rigosertib is a microtubule-destabilizing agent

talk

Kao, Winston W

Gene therapy of mucopolysaccharidosis type VII (MPS VII) with CRISPR/Cas9 genome editing

poster

Kim, Sang Yong

Method for CRISPR-Cas9 gene-editing in egg cells incorporating predictive success

poster

Klann, Tyler S

CRISPR–Cas9 epigenome editing enables high-throughput screening for functional regulatory elements in the human genome

poster

Kocak, Daniel D

Increasing specificity of CRISPR-Cas systems using RNA secondary structure

poster

Koonin, Eugene

Mobile genetic elements and evolution of CRISPR-Cas systems—Both cas genes and spacers come from the mobilome

talk

Kosicki, Michal

Allelic diversity introduced by Cas9

poster

Kousholt, Arne Nedergaard

Developing a CRISPR-CAS9 functional assay approach to classify BRIP1/FANCJ gene variants of uncertain significance

poster

Kulcsar, Peter Istvan

Crossing enhanced and high fidelity SpCas9 nucleases

poster

Le, Thi Thanh Huong

Application of gene therapy for Rett syndrome

poster

Lee, Eun-Seo Ency

In vivo high-throughpu profiling of CRISPR–Cpf1 activity

poster

Lee, Jae Hoon

The PTC-containing mRNAs in the engineered nuclease-mediated knockout mice

poster

Lee, Yoon Hoo

Generation of simultaneous inversion and knock-in based hemophilia mouse model using CRISPR/Cas9

poster

Legube, Gaelle

Chromatin and chromosome dynamics during DNA double strand break repair

talk

Leonetti, Manuel D

Highly specific gene knock-in using long single-stranded DNA donors in human cells

talk

Lim, Kayeong

Genome-wide target specificities of CRISPR RNA-guided programmable deaminases (Assessing and improving the target specificities of CRISPR-mediated base editing)

poster

Lin, Yingbo

High throughput arrayed CRISPR-Cas9 screening for genes involving in IGF-1R SUMOylation dependent cell growth

poster

Liu, David R

Base editing—Genome editing without double-stranded DNA cleavage

talk

Long, Chengzu

Genome editing to model and treat familial dilated cardiomyopathy

poster

Lukyanchikova, Varvara

Directed modifications in structure of topologically associating domains in mice by CRISPR/Cas9-mediated editing

poster

Luttgeharm, Kyle D

Automating CRISPR mutation detection and zygosity determination

poster

Mabuchi, Meg

In vitro characterization of Cpf1 endonucleases as tools for molecular biology

poster

Machado, Hidevaldo B

Chemical modifications of synthetic guide RNA for enhanced RNA stability and reduced cellular toxicity in CRISPR-Cas9 genome editing

poster

Mahiou, Jerome

A CRISPR/Cas9 based gene editing platform to create cell lines expressing CFTR variants from the native gene locus and compatible with functional assay

poster

Maino, Eleonora

Exploring the therapeutic potential of CRISPR/Cas9 technology for the treatment of MeCP2 duplication syndrome

poster

Marco, Eugenio

UDiTaS™—A streamlined genome editing detection method for on- and off-target edits, large deletions, and translocations

poster

Maresca, Marcello

Application of In vivo genome and In vivo base editing in drug discovery

poster

Marraffini, Luciano

How the acquisition of new spacers optimizes targeting during the CRISPR-Cas immune response

talk

Maumenee, Irene

CRB1-related retinal dystrophies are ideal recipients for CRISPR-RNP therapy

poster

McFaline, Jose L

Using combinatorial forward genetics and single cell RNA-seq to determine effectors of spatial signaling during the epithelial to mesenchymal transition

poster

McLaughlin, Gemma M

Gene drives to kill wasps—A novel approach to pest management in New Zealand

poster

Miano, Joe M

CRISPR interrogation of regulatory sequence function in mice

poster

Min, Yi-Li

Correction of hot spot mutations in Duchenne muscular dystrophy (DMD) by CRISPR-Cas9 gene editing

talk

Mittler, Eva-Maria

Using genome-wide CRISPR/Cas9 screens for identification of host factors required for hantavirus entry and infection in human cells

poster

Miyaoka, Yuichiro

Binding affinity of Cas9 to DNA determines its HDR and NHEJ activities

talk

Morell, Montserrat

A PCR based method to create long, single-stranded DNA donors for knockin applications

poster

Muzumdar, Mandar D

Engineering the cancer genome

talk

Nadakuduti, Satya Swathi

Genome wide evaluation of off-targets caused by targeted gene editing in diploid potato

poster

Nakade, Shota

Selective choice of MMEJ repair pathway and its accuracy profile during the process of PITCh knock-in

poster

Nakamae, Kazuki

Automated web-based design tool for MMEJ-mediated gene knock-in

poster

Neggers, Jasper

Target identification using large-scale CRISPR/Cas-mediated mutagenesis scanning of essential genes

poster

Niakan, Kathy

Uncovering mechanisms of early human lineage specification by CRISPR/Cas9-mediated genome editing

talk

Oakes, Benjamin L

Engineering conditional CRISPR molecules to sense and respond to pathogens

talk

Ondrus, Alison E

Metabolic control of the Hedgehog signaling pathway

poster

Petris, Gianluca

Hit and go Cas9 delivered through a lentiviral based self-limiting circuit

poster

Pinello, Luca

CRISPResso2—Ultra-fast quantification of genome editing events for CRISPR-Cas9, Cpf1 and base editors from deep sequencing data

poster

Porteus, Matthew

CRISPR/Cas9 based homologous recombination in primary human blood cells—Advancing the technology and moving to the clinic

talk

Qin, Peter Z

CRISPR-Cas9 mediated DNA unwinding detected using site-directed spin labeling

poster

Riesenberg, Stephan

Targeting repair pathways with small molecules increases targeted nucleotide substitution efficiency in pluripotent stem cells

poster

Robertson, Louise

Expanding the toolkit for mouse genome editing—Analysis of CRISPR variants with different PAM recognition

poster

Ryu, Seuk-Min

Highly efficient RNA-guided base editing in mouse embryos

poster

Saito, Makoto

HDAC6-mediated deacetylation of the DDX3X intrinsically disordered region controls stress granule assembly dynamics

poster

Saunders, Thom

CRISPR/Cas9 genome editing pipeline for mice and rats

poster

Savage, David

High-throughput approaches for engineering CRISPR-Cas proteins

talk

Schneller, Jessica L

In Vivo genome editing to correct a model of methylmalonic acidemia (MMA)

poster

Schraivogel, Daniel

Targeted CRISPR-DropSeq for large-scale functional genomics

poster

Sentmanat, Monica

Validation strategies for CRISPR-Cas9–edited pools

poster

Sfeir, Agnel J

Polymerase theta promotes error-prone repair by alt-NHEJ at the expense of error-free HR

talk

Sharma, Sumana

Genome-wide CRISPR-Cas9 cellular screens to investigate cell surface protein interactions

talk

Sheltzer, Jason

Validating and invalidating cancer drugs and drug targets using CRISPR/Cas9

talk

Shendure, Jay

Multiplex strategies for measuring variant effects

talk

Skor, Maxwell N

Therapeutic correction of an LCA-causing splice defect in the CEP290 gene by CRISPR/Cas-mediated genome editing

talk

Sontheimer, Erik

Type II-C CRISPR-Cas systems and their natural inhibitors

talk

Strezoska, Zaklina

Identification of genes involved in cell cycle regulation using arrayed synthetic CRISPR RNA libraries in a multiparameter high-content assay

poster

Sugano, Shigeo

Development of versatile modules for NHEJ-based knock-in and optimization of the ratio of donor and cleavage vectors

poster

Taghbalout, Aziz

Casilio-ME—A robust platform for targeted DNA demethylation

poster

Tak, Esther Yu Gyoung

Inducible, tunable, and multiplex human gene regulation using CRISPR-Cpf1-based transcriptional activators

talk

Tan, Meng How

Comprehensive evaluation of CRISPR-Cas systems for genome editing in human cells

poster

Tillotson, Eric

A standard method for benchmarking of CRISPR-associated endonuclease activity as RNPs on free DNA matched substrates and in mammalian cells

poster

Tsang, Stephen H

Mechanistic insights into EFEMP1-related Malattia Leventinese disease and implications for dry AMD

poster

Turunen, Mikko

CRISPR- and CRISPRa screens for identification of target genes for allelic imbalance target regions in colorectal cancer

poster

Vakulskas, Christopher

A novel Cas9 mutant confers reduced off-target gene editing while maintaining on-target potency

poster

Waigmann, Elisabeth

EFSA safety assessment of plants obtained through cisgenesis, intragenesis or Site-directed nucleases (SDN-3)

poster

Wang, Chia-Lin

RNAi and CRISPR/Cas9 based in vivo models for drug discovery

poster

Wiedenheft, Blake

A CRISPR immune response to viruses that infect bacteria

talk

Wilson, Ross C

Tissue-specific delivery of CRISPR-Cas9 via receptor-mediated uptake

poster

Winter, Julia

Manipulation of MEF-2 activity in the rat brain using CRISPR/Cas9 and its relevance for anxiety, schizophrenia, or autism

poster

Wozniakowski, Grzegorz J

Isolation, molecular analysis and preparation of African swine fever strains for CRISPR/Cas9 modification

poster

Yang, Jian-Ping

GeneArt arrayed lentiviral CRISPR libraries as powerful high-throughput loss-of-function screening tools

poster

Yuan, Celvie L

Enrichment of CRISPR-mediated homology-directed repair in mammalian cells by cell cycle-based flow sorting

poster

Yudovich, David

Molecular and bioinformatic workflow for robust NGS-based genome editing quantification

poster

Zhang, Yu

CRISPR/Cpf1 correction of muscular dystrophy mutations in human cardiomyocytes and mice

poster