GENOME ENGINEERING: THE CRISPR/CAS REVOLUTION
September 24 - 27, 2015

You must register for the meeting in order to submit abstracts.
After registering you will be sent a web link for abstract submission by email.

Program information: An electronic version of the program abstract book will be sent three working days prior to the first day of the meeting, and hard copies will be available for collection upon your arrival at Cold Spring Harbor Laboratory. First night and keynote speakers are informed of their session date and time, otherwise program information is only available upon release of the electronic version of the abstract book. The reason we do this is to try and maximize interactions by encouraging participants to stay for the duration of the meeting.

Please check your email for talk length, poster instructions, and how to have your poster printed at CSHL for collection upon arrival. 

ABSTRACT STATUS

Presenting Author

Abstract Title

Talk/Poster

Adikusuma, F.

Frequent large deletions generated by targeted CRISPR/Cas9 cleavage indicate a novel DNA repair mechanism

poster

Aida, T.

Cloning-free CRISPR/Cas system facilitates functional cassette knock-in in mice

poster

Akhmetov, A.

CRISPR-mediated humanization of yeast

poster

Ali, Z.

CRISPR/Cas9 system mediates Interference with plant DNA viruses

talk

Anand, R.P.

Non-homologous tail differentially modulate homologous and homeologous recombination

poster

Anderson, D.G.

Nucleic acid delivery systems for RNA therapy and gene editing

talk

Aparicio, E.

DECKO—A simple and multiplexable CRISPR tool for targeted deletion of genomic elements including long noncoding RNAs

poster

Aryal, N.K.

Lessons learned by targeting essential genes using CRISPR/Cas9 system

poster

Baron, G.N.

CRISPR/Cas9-mediated gene editing in Arginase-1 deficient induced pluripotent stem cells

poster

Bauer, D.E.

Cas9-mediated in situ saturating mutagenesis for BCL11A enhancer functional dissection.

talk

Bennett, E.P.

Improved targeting and indel identification methodologies for genome editing

poster

Bernard, A.L.

Use of the CRISPR/Cas9 system to generate simultaneously mutant and GFP transgenic zebrafish for the Foxo1a gene

poster

Bertier, L.D.

Towards generating multi-disease resistant lettuce using Crispr/CAS9 genome editing

poster

Blitz, I.L.

Leapfrogging—A method for targeting CRISPR/Cas9 genome editing to the germline in Xenopus

talk

Boel, A.

CRISPR/Cas-mediated gene editing in zebrafish—An optimized workflow

poster

Bothmer, A.H.

Enhancing CRISPR/Cas9 mediated DNA repair outcomes through the local recruitment of DNA repair factors

poster

Bustos, F.J.

Use of CRISPRs to Remove of a spontaneous mouse mutation causing abnormal anxiety- /autism-like behaviors

poster

Cai, M.

Genome editing to understand the function of CD33 in neuro-inflammation and Alzheimer's disease

poster

Carpenter, M.A.

Editing editors with editing

talk

Charo, R.

Germline editing of plants and non-human animals

talk

Charpentier, E.

CRISPR-Cas9—From a bacterial immune system to a revolution in genome engineering

talk

Cheng, X.

CRISPR/Cas9-based genome modification of a novel rat long non-coding RNA within a homologous GWAS associated locus for QT-intervals

poster

Chiang, W.

CRISPR-Cas9D10A nickase-based genotypic and phenotypic screening to enhance efficient and accurate genome editing

poster

Church, G.M.

Radically new genomes—E.coli, elephants, mosquitoes, pigs, humans.

talk

Cockrell, A.S.

A CRISPR/Cas9 engineered mouse model for the middle east respiratory coronavirus (MERS-CoV)

poster

Conklin, B.

Precision genome editing—Choosing the right nuclease for scarless point mutations in human iPS cells

talk

Corn, J.

Cas9 asymmetrically releases one strand of target DNA, enabling rational design of templates for improved gene editing

talk

Cotta-Ramusino, C.

Repair pathway choice in response to different types of DNA lesions mediated by different CRISPR/Cas9 variants

talk

Courtney, D.G.

The development of allele-specific CRISPR/Cas9 based therapeutics for corneal disorders

poster

Crist, A.B.

A CRISPR-based mating system switch and the experimental evolution of sexual interactions in C. elegans

poster

Cuomo, E.

CRISPR/ Cas gene editing in the study of drug resistance in EGFR- driven lung cancers

poster

Daggubati, V.

Identifying drug resistances using genome-wide CRISPR-CAS9 libraries

poster

Davis, D.J.

An innovative approach for analyzing the effects of stress on the kynurenine pathway in zebrafish

poster

De Rocker, N.

Development of a myt1l knockdown zebrafish model using CRISPR-Cas9

poster

Demozzi, M.

Yeast-based assays for CRISPR/Cas9-mediated homologous recombination

poster

Dickel, D.E.

Systematic deletion screens of distant-acting in vivo enhancers in mice

talk

Dillard, V.

Making the cut—Software for designing a revolution in genome editing

poster

Ding, M.

Proofreading the genome—Towards the development of a CRISPR/Cas9-based therapy for merosin-deficient congenital muscular dystrophy

poster

Ding, X.

Synthetic viability by BRCA2 and PARP1 deficiency

talk

Doyon, Y.

A scalable genome editing-based approach for mapping the human protein interactome

poster

Droguett, G.

Creation of large, single-step homozygous humanizations of mouse genes by combining CRISPR/Cas9 cleavage with BAC-based targeting vectors

poster

Duan, J.

Open chromatin dynamics and genetic perturbation by CRISPR editing in iPSC-derived neurons at the MIR137 schizophrenia risk locus

talk

Ehsaei, Z.

CRISPR targeting of human induced pluripotent stem cells (hiPSCs) for lineage tracing of hiPSCs-derived neural stem cells during cortical development in vitro

poster

Eid, A.

Viral-mediated genome editing in Arabidopsis using the CRISPR/Cas9 system

poster

Federici, F.

Using CRISPR/Cas for morphogenetic engineering of bacterial colonies

poster

Frisch, R.L.

A Non-conventional gene editing method in a non-conventional yeast—Using Cas9 for genome editing in Yarrowia lipolytica

poster

Frock, R.L.

Genome-wide detection of DNA double-stranded breaks induced by engineered nucleases

talk

Fujii, H.

Locus-specific biochemical analysis of genome functions using enChIP—An application of CRISPR/Cas to purification of specific genomic regions

poster

Greely, H.

Human germline genome editing—Breaking out the issues

talk

Guay, D.

Innovative protein delivery system for cell therapy

poster

Gupta, A.

Developing a drug-inducible CRISPR/Cas9 system for precise genome editing

poster

Gurumurthy, C.B.

Efficient insertion of sequences using longer single stranded repair DNAs at the Cas9 cut sites

poster

Guzzardo, P.

Genome editing in haploid human cells using CRISPR/Cas9

poster

Haber, J.

Homologous recombination and nonhomologous end-joining of Cas9-cleaved DNA in budding yeast

talk

Hart, T.

High-resolution detection of fitness genes and genotype-specific cancer vulnerabilities with CRISPR-Cas9 screens

talk

Hendel, A.

Next-generation CRISPR guide RNAs with chemical modifications enhance genome editing in human primary cells

poster

Hernandez Magadan, A.

Matching phenotypes to mutations in industrial bacterial strains by using CRISPR-Cas technology

poster

Hewitt, A.

Public perception of human gene editing—Taking the pulse of the global community’s attitude towards CRISPR

poster

Hill, M.C.

Complementary genome-wide CRISPR-Cas9 screens to characterize the molecular basis for Doxorubicin-induced cardiotoxicity

poster

Hoang, M.

Creating human cellular models to investigate cardioprotective functions of GRK5 Gln41Leu single nucleotide polymorphism using CRISPR/Cas9 technology

poster

Honig, A.

MemoGeneTM enables transient expression of CRISPR components—New possibilities for plant genome editing

poster

Horlbeck, M.A.

Development of next-generation libraries for genome-scale control of gene repression and activation

talk

Hotta, A.

Dual regulation of CRISPR-Cas9 for controlling cleavage activity in iPS cells

poster

Hu, Y.

Selection of guide RNAs for generation of genetically modified mice

poster

Huang, B.

Imaging the genome in living cells

talk

Huang, C.

In vivo discovery of drug targets in liver cancer by RNAi in combination with CRISPR/Cas9

talk

Huangfu, D.

Genome editing in human pluripotent stem cells for understanding the mechanisms of pancreatic development and diabetes

talk

Jacobs, E.Z.

CRISPR/Cas9-mediated genome editing in primed versus naïve human embryonic stem cells

poster

Jae, L.T.

Studying genetic interactions in nuclease-engineered haploid human cells

talk

Josling, G.

Dissecting the regulation of malaria transcription factors using CRISPR/Cas9

talk

Joung, J.

Defining, improving, and altering the specificities of CRISPR-Cas9 nucleases

talk

Julien, D.P.

Identifying zebrafish somatosensory subtypes using CRISPR/Cas9-directed transgene integration

poster

KaramiNejadRanjbar, M.

Adapting Cas9 mediated genome editing for genome wide arrayed screening in cancer cell lines

poster

Kiani, S.

Mammalian genetic kill switches using Cas9 gRNA systems

poster

Kim, D.

Multiplexed Digenome-seq: Genome-wide profiling of CRISPR-Cas9 off-target effects in human cells

poster

Kim, H.

Blood group conversion using transcription activator-like effector nucleases

poster

Kim, J.

Genome editing in stem cells, animals, and plants

talk

Kim, S.

Generation of genome-modified mice by microinjection of CRISPR/Cas9 using sperm and egg banking

poster

Knight, S.C.

Single-molecule dynamics of CRISPR-Cas9 genome interrogation in living cells

talk

Knowlton, M.N.

Data integration through use of nomenclature and biological ontologies—Naming and annotating TALENs, ZFNs and CRISPR/Cas mutations in mice

poster

Koeferle, A.

Forward epigenetics—A CRISPR-based screening method

poster

Kosicki, M.

Generating megabase size deletions in mouse embryonic stem cells using CRISPR/Cas9

poster

Kueh, A.

HBO1 is essential for H3K14 acetylation in human cell lines

poster

Kumar, S.

Highly efficient genome editing and cell engineering in stem cells using CRISPR/Cas9

poster

Kurata, J.

Determining microRNA function by gene knockout using TALEN or CRISPR-Cas9 based screens

poster

le Sage, C.

CRISPR-Cas9D10A nickase-based genotypic and phenotypic screening to enhance efficient and accurate genome editing

talk

Lee, C.M.

Optimization of CRISPR/Cas9 systems for large genomic deletions

poster

Lee, J.

Customized site specific DNA cloning method based on type-II CRISPR/Cas bacterial immune system and isothermal assembly

poster

Lee, K.

Gold nanoparticle-mediated delivery of CRISPR/Cas9 ribonucleoprotein and DNA donor for efficient genome editing in vitro and in vivo

poster

Lee, W.

Construction of CRISPR/Cas DNA molecules based on microchip oligonucleotides and next-generation sequencing technology

poster

Li, W.

Quality control, modeling and visualization of genome-wide CRISPR screens using MAGeCK-VISPR

talk

Long, C.

Rescue of Duchenne muscular dystrophy cardiomyocyte function by myoediting

talk

Ma, H.

Live cell interrogation of CRISPR dynamics with fluorescent sgRNAs

poster

Maizels, N.

Genome engineering at targeted DNA nicks

talk

Mansouri, M.

MultiPrime—A baculovirus-based multigene expression system for mammalian cells

poster

Marraffini, L.

Tolerance during CRISPR-Cas immunity—Domesticating the virus

talk

Martens, A.

Investigating A20’s (non-)enzymatic activities in vivo through CRISPR-Cas gene targeting

poster

Martins, N.

Establishment of a novel model for the Nup98-HoxA9 leukemogenic translocation using CRISPR/Cas9

poster

Mashimo, T.

CRISPR/Cas9 with ssODNs enables gene knock-in and replacement of large genomic regions in rats

poster

Michealraj, A.

Modelling medulloblastoma using the CRISPR/Cas9 system to generate large chromosomal deletions in mice

poster

Mohr, G.

A reverse transcriptase-Cas1 fusion protein mediates site-specific integration of both RNA and DNA spacers into CRISPR repeats

talk

Morell Fernandez, M.

Direct delivery of Cas9 protein via cell-derived nanovesicles

poster

Moreno-Mateos, M.A.

CRISPRscan—Designing highly efficient sgRNAs for CRISPR/Cas9 targeting in vivo

poster

Nakanishi, T.

Efficient DNA integration into germ line genome via programmable nucleases in Daphnia magna

talk

Natsume, T.

A PCR-based tagging method for generation of human conditional mutants by use of the auxin-inducible degron technology

poster

Neggers, J.

A single amino acid substitution by CRISPR/Cas9 genome editing at cysteine 528 of XPO1 confers resistance of malignant cells to SINE compounds

poster

Oliveira, R.

Generation of Dmpk knockin mice with large CTG repeat expansions to model myotonic dystrophy

poster

Ortiz, O.

Optimization of CRISPR/Cas9 components for gene therapy

talk

Paix, A.

Homology-directed genome editing in C. elegans—Systematic analysis of parameters yields a high efficiency, cloning and marker-free protocol

talk

Parts, L.

Multiplex evaluation of programmable CRISPR/Cas9 transcription factors using competitive growth assays in yeast

talk

Peterson, K.A.

CRISPR/Cas9 mediated gene modification provides a robust platform for modeling developmental disorders

poster

Petris, G.

CRISPR/Cas9 viral like particles for targeted genome editing in human cells

poster

Piatek, A.A.

RNA-guided transcriptional regulation in planta via synthetic dCas9-based transcription factors

poster

Pinello, L.

CRISPResso—Analysis of CRISPR-Cas9 genome editing outcomes from deep sequencing data

poster

Prakash, R.

Nuclease-generated mouse mutants of homologous recombination genes

talk

Qi, L.

CRISPR/Cas9 for transcription regulation and genome imaging

talk

Qin, L.

CRISPR-Cas9 delivery to hard-to-transfect cells via membrane deformation

poster

Qin, W.

Genome editing in mice mediated by the CRISPR/Cas9 nuclease with high efficiency and throughput

poster

Rahman, S.H.

CRISPR/Cas9-mediated targeted knockout of protein S in Chinese hamster ovary (CHO) cells

poster

Ranganathan, V.

In vivo genome editing mediated by co-delivery of SpCas9 and guide RNA through a single AAV

poster

Ratz, M.

Live cell RESOLFT super-resolution microscopy of endogenously tagged human cells

poster

Ravinder, N.

Rapid and highly efficient cell engineering with Cas9 ribonucleoproteins and lentiviral CRISPR systems

poster

Reha, S.

CRISPR/Cas-based nucleases and nickases for targeted mutagenesis and HR-mediated gene targeting in plants

poster

Richie, C.T.

Development of Cre-dependent AAV CRISPR toolkit

poster

Richter, T.R.

The Icelandic transgenic core facility as a platform to apply the CRISPR technology

poster

Rose, J.C.

Development and application of a chemically inducible Cas9

poster

Rozwadowski, K.L.

In vivo production of multicopy donor template for gene targeting using retron-mediated reverse transcription

poster

Ruiz, S.

Cas9/RNA-based forward genetic screenings in mouse embryonic stem cells uncovered the role of genes mediating resistance to ATR inhibitors

talk

Sadhu, M.J.

Genetics without crosses—Quantitative trait mapping with CRISPR

poster

Scaffa, A.M.

Engineering splicing mutations in HEK 293 cells using CRISPR/Cas9 system

poster

Schmid-Burgk, J.

CRISPaint—Ligase-4 dependent modular gene tagging

poster

Serebrenik, A.A.

Functional interaction between the cancer genomic DNA deaminase APOBEC3B and uracil excision repair

poster

Sharma, S.

CRISPR-Cas9 based cellular screens to identify novel low-affinity extracellular protein interactions

poster

Shi, J.

A domain-focused CRISPR strategy for cancer drug target discovery

talk

Shirole, N.

A rapid and tunable method to temporally control Cas9 expression enables the characterization of essential genes and the interrogation of functional gene interactions in vitro and in vivo

poster

Skarnes, W.C.

Biallelic genome editing of human pluripotent stem cells at scale

talk

Smemo, S.

Determining causal alleles in age-related macular degeneration

poster

Smith, A.J.

Design considerations for highly specific and efficient synthetic crRNA molecules

poster

Sontheimer, E.J.

Multimeric guides and tracrRNA-independent Dnase H activity for Neisseria meningitidis Cas9

talk

Speleman, F.

CRISPR-Cas9 modeling of cell lines and zebrafish to study the TLX1-PHF6 cooperative interrelationship in T-cell acute lymphoblastic leukemia

talk

St.Onge, B.P.

A scalable method for isolating sequence-verified oligonucleotides from complex libraries

poster

Svitashev, S.K.

Maize genome editing using Cas9-guideRNA technology

talk

Symington, L.

Making the right choice at a DNA double-strand break

talk

Takeda, J.

A combination of Bloom gene inactivation and CRISPR/Cas9-mediated DNA cleavage facilitates construction of a loss-of-heterozygosity library of the mammalian genome

poster

Thurlow, L.A.

A fluorescently labelled dCas9 for imaging endogenous RNAs in vivo

poster

Toki, S.

Establishment of an efficient CRISPR/Cas9 mediated genome editing system in rice

poster

Tsai, S.

Genome-wide specificities of wild-type and engineered CRISPR-Cas9 nucleases defined by GUIDE-seq

poster

Tsang, S.H.

CRISPR/Cas9 gene-editing of genome-wide association (GWAS) alleles in patient specific stem cell lines

talk

Turunen, M.

Lineage-specific oncogenic transcription factors of different cancer types converge to regulate the activity of MYC and CDK4/6

poster

Urnov, F.D.

Genome editing in primary human cells and organs—Towards the goal of engineering genetic cures

talk

Varshney, G.K.

High throughput gene targeting in zebrafish using CRISPR/Cas9

talk

Vasileva, A.

Human cell line engineering for enhanced efficiency of CRISPR/Cas9-mediated genome editing

poster

Ventura, A.

In vivo modeling of chromosomal rearrangements using CRISPR/Cas9

talk

Waghulde, H.

Development of a novel Gper-1 knock out rat model using a modified CRISPR/Cas9 technology

poster

Wang, Q.

Genome-wide identification of CRISPR/CAS9 target sites with reduced off-target effects in organisms with different genome sizes

poster

wang, X.

Heritable gene disruption in goats with CRISPR/Cas9 results in phenotypic changes

poster

Watters, K.E.

Structure-function design principles for CRISPR small guide RNAs

poster

Wienert, B.

Editing the genome to introduce a beneficial naturally occurring mutation associated with increased fetal globin

talk

Wiggins, C.

CRISPRevolution—New opportunities in target identification and validation

poster

Willaert, A.

Modeling the arterial tortuosity syndrome (ATS) in zebrafish using CRISPR/Cas genome editing

poster

Winters, I.P.

Pancreatic cancer modeling with viral vectors and in vivo CRISPR/Cas9-mediated somatic genome editing

talk

Wolfe, S.

Improving CRISPR/Cas9 Precision through tethered DNA-binding domains

poster

Wu, M.

Type I CRISPR-Cas systems target endogenous genes to dampen host immunity

poster

Wu, Q.

CRISPR inversion of CTCF binding sites alters genome topology and enhancer/promoter function

talk

Wu, R.

Interactions among resistance genes lead to hybrid necrosis

poster

Wu, Y.

Lineage-specific report lines generated by double nicking CRIPSR/Cas9 in human embryonic stem cells

poster

Xiong, K.

Synergistic activation mediator (SAM) induced expression of reprogramming genes in porcine fibroblast

poster

Xue, W.

CRISPR-mediated direct mutation of cancer genes in the mouse liver

talk

Yeganeh Seghalaksari, M.

CRISPR/Cas9-mediated deletion of a SINE element within the Polr3e gene reveals a role in gene expression regulation

poster

Yuan, Y.

Crystal structures of CRISPR-associated Csx3 reveal a manganese-dependent deadenylation exoribonuclease

poster

Yudovich, D.

CRISPR-Cas9 mediated regulation of human hematopoietic stem and progenitor cells

poster

Yusa, K.

A genome-wide CRISPR-Cas9 screen identifies genetic vulnerability in acute myeloid leukaemia

talk

Zerbini, F.

Genome editing in Escherichia coli via CRISPR-Cas9 system

poster

Zhang, F.

Genome editing using CRISPR-Cas9

talk

Zhang, H.

CRISPR/Cas-mediated gene editing in human iPSC-derived macrophage reveals lysosomal acid lipase function in human macrophages

talk

Zhongbao, Z.

Isolation, structural analysis, and expression characteristics of the maize TIFY gene family

poster

Zuckermann, M.

Somatic CRISPR/Cas9-mediated tumor suppressor disruption enables versatile brain tumor modeling

talk