YEAST
GENETICS & GENOMICS
July 24- August 13, 2007
Application Deadline: March 15, 2007
Instructors:
Beverly
Errede, University of North Carolina Chapel Hill
Frank
Luca, University of Pennsylvania
Malcolm
Whiteway, National Research Council of Canada
The
Yeast Genetics & Genomics course is a modern, state
of the art laboratory course designed to teach the students
the full repertoire of genetic approaches needed to dissect
complex problems in the yeast Saccharomyces cerevisiae.
Combinations of classical genetic approaches are emphasized,
including the isolation and characterization of mutants,
tetrad analysis, complementation, and mitotic recombination.
Molecular genetic techniques, including various types of
yeast transformation, gene replacement with plasmids and
PCR, construction and analysis of gene fusions, and generation
of mutations in cloned genes, will also be emphasized. Students
will use classical and molecular approaches to gain experience
in identifying and interpreting various kinds of genetic
interactions including suppression and synthetic lethality.
Students will be immersed in yeast genomics and will perform
and interpret experiments with DNA arrays. Students will
gain first-hand experience in modern cytological approaches
such as epitope tagging and imaging yeast cells using indirect
immunofluorescence, GFP-protein fusions and a variety of
fluorescent indicators for various subcellular organelles.
Lectures on fundamental aspects of yeast genetics will be
presented along with seminars given by outside speakers
on topics of current interest.
Last
year's speakers included:
David Amberg, SUNY Upstate Medical
University
Linda Breeden, Fred Hutchinson Cancer Research Center
Dan Burke, University of Virginia
Kara Dolinski, Princeton University
Beverly Errede, University of North Carolina Chapel Hill
Daniel Gottschling, Fred Hutchinson Cancer Research Center
Michael Gustin, Rice University
Alan Hinnebusch, NICHD, NIH
Chris Kaiser, Massachusetts Institute of Technology
James Konopka, SUNY Stony Brook
Rodney Rothstein, Columbia University College of Phys. &
Surgs.
Pamela Silver, Dana Farber Cancer Institute/Harvard University
Michael Snyder, Yale University
George Sprague, University of Oregon
This
course is supported with funds provided by the Howard
Hughes Medical Institute
Cost
(including board and lodging): $3,690
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