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CELL
& DEVELOPMENTAL
BIOLOGY OF XENOPUS
April 17 - 28, 2009
Application Deadline: January 30, 2009
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Xenopus
is the leading vertebrate model for the study of gene function
in development. The combination of lineage analysis, gene-knockout
strategies, experimental manipulation of the embryo, and genomic/bioinformatic
techniques, makes it ideal for studies on the molecular control
of embryo patterning, morphogenesis and organogenesis. The
course combines intensive laboratory training with daily lectures
from recognized experts in the field. Students will learn
both emerging technologies and classical techniques to study
gene function in Xenopus development. An important
element will be the informal interaction between students
and course faculty.
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Technologies to be covered will include: oocyte and embryo culture,
lineage analysis and experimental manipulation of embryos, time
lapse imaging of morphogenesis, gain and loss of function analysis
using mRNAs and antisense oligos, whole mount in situ hybridization,
immunocytochemistry, RT-PCR, and genomic/bioinformatic techniques,
preparation of transgenic embryos, and use of Xenopus tropicalis
for genetic analyses. The Cell and Developmental Biology
of Xenopus course is designed for those new
to the Xenopus field, as well as for those wanting
a refresher course in the emerging technologies. The course
is open to investigators from all countries.
Photos courtesy of Rob Grainger, Lyle Zimmerman
and Lance Davidson. |
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Lecturers
and assistants for the 2008 included:
Ken Cho, University of California, Irvine
Jan Christian, Oregon Health and Sciences University
Rob Grainger, University of Virginia
Richard Harland, University of California, Berkeley
Janet Heasman, Cincinnati Children's Hospital Res Foundation
Mustafa Khokha, Yale University School of Medicine
Paul Krieg, University of Arizona College of Medicine
Takuya Nakayama, University of Virginia
Christopher Wylie, Cincinnati Children's Hospital Res Foundation
This
course is supported with funds provided by the National
Institute of Child Health and Human Development, the National
Science Foundation and the Howard
Hughes Medical Institute
Cost
(including board and lodging): $2,915
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