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CELL & DEVELOPMENTAL
BIOLOGY OF XENOPUS
April 17 - 28, 2009
Application Deadline: January 15, 2009

Instructors:
Raymond Keller, University of Virginia
Kristen Kroll, Washington University School of Medicine

Xenopus is the leading vertebrate model for the study of gene function in development. The combination of lineage analysis, gene-knockout strategies, experimental manipulation of the embryo, and genomic/bioinformatic techniques, makes it ideal for studies on the molecular control of embryo patterning, morphogenesis and organogenesis. The course combines intensive laboratory training with daily lectures from recognized experts in the field. Students will learn both emerging technologies and classical techniques to study gene function in Xenopus development. An important element will be the informal interaction between students and course faculty.
Technologies to be covered will include: oocyte and embryo culture, lineage analysis and experimental manipulation of embryos, time lapse imaging of morphogenesis, gain and loss of function analysis using mRNAs and antisense oligos, whole mount in situ hybridization, immunocytochemistry, RT-PCR, and genomic/bioinformatic techniques, preparation of transgenic embryos, and use of Xenopus tropicalis for genetic analyses. The Cell and Developmental Biology of Xenopus course is designed for those new to the Xenopus field, as well as for those wanting a refresher course in the emerging technologies. The course is open to investigators from all countries.

Photos courtesy of Rob Grainger, Lyle Zimmerman and Lance Davidson.

Lecturers and assistants for the 2008 course will include:
Ken Cho, University of California, Irvine
Jan Christian, Oregon Health and Sciences University
Rob Grainger, University of Virginia
Richard Harland, University of California, Berkeley
Janet Heasman, Cincinnati Children's Hospital Res Foundation
Mustafa Khokha, Yale University School of Medicine
Paul Krieg, University of Arizona College of Medicine
Takuya Nakayama, University of Virginia
Christopher Wylie, Cincinnati Children's Hospital Res Foundation

This course is supported with funds provided by the National Institute of Child Health and Human Development, the National Science Foundation and the Howard Hughes Medical Institute

Cost (including board and lodging): $2,720
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