CELL
& DEVELOPMENTAL BIOLOGY OF XENOPUS
April 14 - 24, 2007
Application Deadline: January 15, 2007
Instructors:
Janet
Heasman & Christopher
Wylie,
Cincinnati Children's Hospital Research Foundation
Xenopus
is the leading vertebrate model for the study of gene function
in development. The combination of lineage analysis, gene-knockout
strategies, experimental manipulation of the embryo, and genomic/bioinformatic
techniques, makes it ideal for studies on the molecular control
of embryo patterning, morphogenesis and organogenesis. The
course combines intensive laboratory training with daily lectures
from recognized experts in the field. Students will learn
both emerging technologies and classical techniques to study
gene function in Xenopus development. An important
element will be the informal interaction between students
and course faculty.
Technologies
to be covered will include: oocyte and embryo culture, lineage
analysis and experimental manipulation of embryos, gain and
loss of function analysis using mRNAs and antisense oligos,
whole mount in situ hybridization, immunocytochemistry, RT-PCR,
and genomic/bioinformatic techniques, preparation of transgenic
embryos, and use of Xenopus tropicalis for genetic analyses.
The Cell and Developmental Biology of Xenopus
course is designed for those new to the Xenopus
field, as well as for those wanting a refresher course in
the emerging technologies. The course is open to investigators
from all countries.
Additional
lecturers in the 2006 course included: Stefano
Piccolo, Ray Keller, Tim Grammer, Carole LaBonne, Tim Mohun,
Aaron Zorn and Matt Kofron.
This course is supported with
funds provided by the
National Institute of Child Health and Human Development,
the National
Science Foundation and the Howard
Hughes Medical Institute
Cost
(including board and lodging): $2,605
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