This intensive laboratory and
lecture course will focus on cutting-edge proteomics approaches
and technologies. In the protein profiling portion of the
course, students will gain hands-on experience in several
quantitative proteome analysis methods, including two-dimensional
gel electrophoresis and isotopic labeling strategies. Students
will be trained to use DIGE, or differential in-gel electrophoresis,
for gel-based protein quantification. Differentially expressed
proteins will be determined using advanced gel analysis software,
and identified using MALDI mass spectrometry. Students will
be taught differential and quantitative mass spectrometry
approaches to profile and identify changes in proteomes. Students
will be trained in high-sensitivity microcapillary liquid
chromatography coupled with nanospray-ESI and tandem mass
spectrometry. Both single dimension and multidimensional microcapillary
liquid chromatography separations coupled to mass spectrometry
will be taught. Students will gain hands-on experience purifying
and identifying protein complexes and post-translational modifications
using the latest technologies. A strong emphasis will be placed
on data analysis throughout the course. The overall aim of
the course is to provide each student with the fundamental
knowledge and hands-on experience necessary to be able to
perform and analyze proteomics experiments, and to learn to
identify new opportunities in applying proteomics approaches
to his/her own research.
Speakers in the 2008 course
included:
Roger Biringer, Life Sciences
Mass Spectrometry
Steven Carr, Broad Institute of Harvard and MIT
Brian Chait, Rockefeller University
Pierre Chaurand,Vanderbilt University School of Medicine
Karl Clauser, Broad Institute of Harvard & MIT
Ileana Cristea, Princeton University
Paul Huang, MIT
Darryl Pappin, Applied Biosystems (now CSHL)
Michael Snyder, Yale University
This
course is supported with funds provided by the National
Cancer Institute
Cost
(including board and lodging): $3,245
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