This intensive laboratory
and lecture course will focus on two major themes in proteomics:
protein profiling and functional proteomics. In the profiling
section of the course, students will learn about cutting-edge
protein separation methods, including hand-on experience with
2-D gel electrophoresis, multidimensional liquid chromatography
and affinity purification of protein complexes. The course
will cover both MALDI and ESI high-sensitivity mass spectrometry
including peptide mass mapping and tandem mass spectrometry,
quantification, and phosphoproteomics. Students will learn
to use several informatics tools available for analyzing the
data. In the functional proteomics section of the course,
students will learn about recombinational cloning, high-throughput
protein isolation, and protein microarrays. Students will
use robots to execute high-throughput methods including expression,
purification and characterization of proteins. They will also
print and analyze their own self-assembling protein microarrays,
which will be used for protein-protein interaction studies.
The overall aim of the course is to provide each student with
the fundamental knowledge and hands-on experience necessary
to be able to perform and analyze proteomics experiments,
and to learn to identify new opportunities in applying proteomics
approaches to his/her own research.
Speakers in the 2005 course
included:
Steven Carr, Broad Institute of Harvard and
MIT
Brian Chait, The Rockefeller University
Pierre Chaurand, Vanderbilt University School of Medicine
Kevin Coombes, MD Anderson Cancer Center
Wade Hines, Beyond Genomics, Inc.
Peter Juhasz, BG Medicine, Inc.
Akhilesh Pandey, Johns Hopkins University
Darryl Pappin, Applied Biosystems, Inc.
Michael Snyder, Yale University
Forest White, Massachusetts Institute of Technology
This
course is supported with funds provided by the National
Cancer Institute
Cost
(including board and lodging): $2,800
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