This
intensive laboratory and lecture course is designed
for biologists interested in using mouse models
to study mammalian development, stem cells and
cancer. Lectures provide the conceptual basis
for contemporary research in embryogenesis,
organogenesis, embryonic, adult and induced
pluripotent stem cells and cancer biology. Laboratory
practicals provide extensive hands-on introduction
to engineering of mouse models, phenotyping
and stem cell technologies. Experimental techniques
to be covered include isolation, in vitro culture
and manipulation of pre- and post-implantation
embryos, embryo transfer, genetic manipulation
of embryonic stem cells, production of chimeras
by embryo aggregation and by embryonic stem
cell injection, and transgenesis by pronuclear
microinjection. The course will also introduce
the generation and differentiation of embryonic
stem cells as well as induced pluripotent stem
cells, isolation of mouse embryonic fibroblasts,
focus formation assay, teratoma formation assay,
time-lapse microscopy of early embryos and organ
cultures. In addition, this year's practicals
will feature increased emphasis on phenotypic
analysis of mutants, including vibratome and
cryosectioning, in situ hybridization, immunostaining,
skeletal preparation, tissue recombination,
confocal and ultrasound imaging.
2011 Speakers:
Richard Behringer, The University of Texas/M.D.
Anderson Cancer Center
Blanche Capel, Duke University Medical Center
Ann Foley, Weill Cornell Medical College
Alexandra Joyner, Sloan Kettering Cancer Center
Monica Justice, Baylor College of Medicine
Robin Lovell-Badge, MRC National Institute for
Medical Research
Terry Magnuson, UNC-Chapel Hill
Andrew McMahon, Harvard University
Andras Nagy, Samuel Lunenfeld Research Institute,
Mt. Sinai Hospital
Larysa Pevny, University of North Carolina
Elizabeth Robertson, University of Oxford
Janet Rossant, The Hospital for Sick Children
Michael Shen, Columbia University Medical Center
Davor Solter, Institute of Medical Biology
Philippe Soriano, Mount Sinai School of Medicine
Lori Sussel, Columbia University
Patrick Tam, Children's Medical Research Institute
Paul Trainor, Stowers Institute for Medical
Reseach
Daniel Turnbull, New York University Medical
Center
