MOLECULAR EMBRYOLOGY OF THE MOUSE
June 3 - 23, 2009
Application Deadline: March 15, 2009

Instructors:
Larysa Pevny, University of North Carolina
Jaime Rivera-Perez, University of Massachusetts Medical School

Visiting Instructors:
Richard Behringer, UT M.D. Anderson Cancer Center
Robin Lovell-Badge, MRC National Inst. for Med. Research, UK
Patrick Tam, Children's Medical Research Institute
David Threadgill, UNC, Chapel Hill
Paul Trainor, Stowers Institute for Medical Research

Co-Instructor:
Kat Hadjantonakis, Memorial Sloan Kettering Cancer Center

Speakers:
Kathryn Anderson, Sloan-Kettering Institute
Blanche Capel, Duke University Medical Center
Frank Costantini, Columbia University, College of P & S
Konrad Hochedlinger, Massachusetts General Hospital
Monica Justice, Baylor College of Medicine
Terry Magnuson, UNC, Chapel Hill
Luis Parada, UT Southwestern Medical Center
Michael Shen, Columbia University Medical Center
Davor Solter, Institute of Medical Biology, Singapore
Philippe Soriano, Fred Hutchinson Cancer Research Center

This intensive laboratory and lecture course is designed for biologists interested in applying their expertise to the study of mouse development. Lectures provide the conceptual basis for contemporary research in mouse embryogenesis and organogenesis, while laboratory practicals provide extensive hands-on introduction to mouse embryo analysis. Experimental techniques to be covered include in vitro culture and manipulation of pre- and post-implantation

embryos, embryo transfer, culture and genetic manipulation of embryonic stem cells, production of chimeras by embryo aggregation and by ES cell injection, and transgenesis by pronuclear microinjection. In addition, this year's practicals will feature increased emphasis on phenotypic analysis of mutants, including techniques of histology, in situ hybridization, immunohistochemistry, skeletal preparation, organ culture, and tissue recombination. The course will also introduce the generation of iPS cells (induced pluripotent cells) and time-lapse microscopy of early gastrulation embryos and organ cultures (eye, kidney and gut).

This course is supported with funds provided by the National Cancer Institute