embryos, embryo transfer, culture and genetic manipulation
of embryonic stem cells, production of chimeras
by embryo aggregation and by ES cell injection,
and transgenesis by pronuclear microinjection. In
addition, this year's practicals will feature increased
emphasis on phenotypic analysis of mutants, including
techniques of histology, in situ hybridization,
immunohistochemistry, skeletal preparation, organ
culture, and tissue recombination.
Seminar
speakers last year included:
Richard Behringer, UT MD Anderson
Cancer Center
Kat Hadjantonakis, Memorial Sloan Kettering Cancer
Center
Konrad Hochedlinger, Harvard Medical School
Monica Justice, Baylor College of Medicine
Robin Lovell-Badge, MRC NIMR, UK
Terry Magnuson, UNC-Chapel Hill
Mark Majesky, University of North Carolina
Anne McLaren, University of Cambridge, UK
Andy McMahon, Harvard University
Peter Mombaerts, The Rockefeller University
Andras Nagy, Samuel Lunenfeld Research Institute,
Mt. Sinai Hospital, Canada
David Ornitz, Washington University
Luis Parada, UT Southwestern Medical Center
Samuel Pfaff, The Salk Institute
Nadia Rosenthal, European Molecular Biology Laboratory,
Italy
Charles Sherr, HHMI/St. Jude Children's Res. Hosp.
Davor Solter, Max-Planck-Institut Fur Immunbiologie,
Germany
Philippe Soriano, Fred Hutchinson Cancer Research
Center
Colin Stewart, National Cancer Institute
Shahragim Tajbakhsh, Pasteur Institute, France
Joseph Takahashi, HHMI/Northwestern University
Patrick Tam, Children's Medical Research Institute,
Australia
Richard Woychik, The Jackson Laboratory