ADVANCED
BACTERIAL GENETICS
June 9 - 29, 2010
Application Deadline: March 15, 2010
Instructors:
John
Kirby, University of Iowa
Susan
Lovett, Brandeis University
Anca
Segall, San Diego State University
DO
YOU NEED (MORE) TRAINING IN BACTERIAL GENETICS?
The
Advanced Bacterial Genetics course presents logic and methods
used in the genetic dissection of complex biological processes
in diverse bacteria. Laboratory methods include: classical
mutagenesis using transposons, mutator strains, and chemical
and physical mutagens; detection and quantitation of gene
expression changes using various reporter genes and real-time
PCR; the mapping of mutations using genetic and physical
techniques; modern approaches to the generation and analysis
of targeted gene disruptions and fusions using PCR and cloning
methods; epitope insertion mutagenesis; site-directed mutagenesis;
and fluorescence microscopy. Key components of the course
will be the use of sophisticated genetic methods in the
analysis of model bacteria (including E. coli,
Salmonella, Bacillus subtilis, and Myxococcus
xanthus), and the use of the wealth of new genomic
sequence information to motivate these methods.
Invited
lecturers will present various genetic approaches to study
bacterial mechanisms of metabolism, development, and pathogenesis.
Speakers
in the 2009 course included:
Thomas
Bernhardt, Harvard Medical School
Jonathan Dworkin, Columbia University
Zemer Gitel, Princeton University
Mark Goulian, University of Pennsylvania
Carol Gross, University of California, San Francisco
Kelly Hughes, University of Utah
Stanley Maloy, San Diego State University
Kit Pogliano, University of California,
San Diego
The course admits 16 students, both foreign
and US residents, from diverse backgrounds and career levels
for intensive (but fun) instruction in microbial genetics.
For
further academic information about the course, feel free
to contact the instructors via their home pages above.
This
course is supported with funds provided by the National
Science Foundation.
