The Yeast Genetics & Genomics course is a modern, state of the art laboratory course designed to teach the students the full repertoire of genetic approaches needed to dissect complex problems in the yeast Saccharomyces cerevisiae. Combinations of classical and modern genetic approaches are emphasized, including the isolation and characterization of mutants, tetrad analysis, complementation, and mitotic recombination as well as array, next generation sequencing, and genome-based methods of analysis facilitated by the yeast genome, the deletion collection, and other genomic resources available to the community. Molecular genetic techniques, including yeast transformation, gene replacement by PCR, construction and analysis of gene fusions, and generation of mutations, will also be emphasized. Students will use classical approaches and modern whole genome sequencing to gain experience in identifying and interpreting various kinds of genetic interactions including suppression and synthetic lethality (including SGA). Students will be immersed in yeast genomics and will perform and interpret experiments to using DNA arrays and multiplex sequencing. Students will gain first-hand experience in modern cytological approaches such as epitope tagging and imaging yeast cells using indirect immunofluorescence, GFP-protein fusions and a variety of fluorescent indicators for various subcellular organelles. Lectures on fundamental aspects of yeast genetics will be presented along with seminars given by prominent experts in the field on topics of current interest.
speakers for 2014 are Maria Costanzo, David Drubin, Scott Emr, Amy Gladfelter, Dan Gottschling, Steve Haase, Sue Jaspersen, Jay Keasling, John McCusker, Chad Myers, Oliver Rando, Rodney Rothstein, Virginia Zakian.