YEAST GENETICS & GENOMICS
July 23 - August 12, 2013
Application Deadline: April 15, 2013

Instructors:

Maitreya Dunham, University of Washington
Marc Gartenberg, Robert Wood Johnson Medical School
Sue Jaspersen, Stowers Institute for Medical Research

See the roll of honor - who's taken the course in the past

The Yeast Genetics & Genomics course is a modern, state of the art laboratory course designed to teach the students the full repertoire of genetic approaches needed to dissect complex problems in the yeast Saccharomyces cerevisiae. Combinations of classical and modern genetic approaches are emphasized, including the isolation and characterization of mutants, tetrad analysis, complementation, and mitotic recombination as well as array, next generation sequencing, and genome-based methods of analysis facilitated by the yeast genome, the deletion collection, and other genomic resources available to the community. Molecular genetic techniques, including yeast transformation, gene replacement by PCR, construction and analysis of gene fusions, and generation of mutations, will also be emphasized. Students will use classical approaches and modern whole genome sequencing to gain experience in identifying and interpreting various kinds of genetic interactions including suppression and synthetic lethality (including SGA). Students will be immersed in yeast genomics and will perform and interpret experiments to using DNA arrays and multiplex sequencing. Students will gain first-hand experience in modern cytological approaches such as epitope tagging and imaging yeast cells using indirect immunofluorescence, GFP-protein fusions and a variety of fluorescent indicators for various subcellular organelles. Lectures on fundamental aspects of yeast genetics will be presented along with seminars given by prominent experts in the field on topics of current interest.

Confirmed speakers include:
Rob Nash, Stanford University: Using SGD
Charlie Boone, University of Toronto: The genetic landscape of a cell
Gerry Fink, Whitehead Institute, MIT: TBD
Fred Winston, Harvard Medical School: Analysis of transcription and chromatin structure in S. cerevisiae and S. pombe
Andrew Murray, Harvard University: Checkpoint and mitosis/Experimental evolution
Jan Skotheim, Stanford University: Single-cell analysis & microfluidics as applied to yeast/cell cycle dynamics
Audrey Gasch, University of Wisconsin-Madison: Genomic analysis of yeast environmental stress responses
Mark Johnston, University of Colorado-Denver: Feasting, fasting & fermenting: glucose sensing by yeasts
Jef Boeke, Johns Hopkins University School of Medicine: Synthesizing and scrambling Sc2.0, a designer yeast genome
Sue Biggins, Fred Hutchinson Cancer Research Center: How do cells get the right chromosomes?
Jeff Smith, University of Virginia: Yeast as a Model System for Aging Research

We anticipate this course will be supported with funds provided by National Science Foundation and the Howard Hughes Medical Institute

Cost (including board and lodging): $4,545
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