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Xenopus is the leading
vertebrate model for the analysis of gene function in
development. The combination of lineage analysis, gene-knockout
strategies, experimental manipulation of the embryo, and
genomic/bioinformatic techniques,
makes it ideal for studies on the molecular control of
embryo patterning, morphogenesis and organogenesis. Moreover,
recent advances in Xenopus genomics offer new opportunities
to integrate computational strategies with experimental
approaches. The course combines intensive laboratory training
with daily lectures from recognized experts in the field.
Students will learn both emerging technologies and classical
techniques to study gene function in Xenopus development.
An important element will be the informal interaction
between students and course faculty.
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Technologies to be covered will include: oocyte and embryo
culture, lineage analysis and experimental manipulation
of embryos, time lapse imaging of morphogenesis, gain
and loss of function analysis using mRNAs and antisense
oligonucleotides, whole mount
in situ hybridization, immunocytochemistry, genomics and
bioinformatics, chromatin immunoprecipitation, preparation
of transgenic embryos, and use of Xenopus tropicalis
for genetic analyses. The Cell
and Developmental Biology of Xenopus course is designed
for those new to the Xenopus field,
as well as for those wanting a refresher course in the
emerging technologies. The course is open to investigators
from all countries.
Photos courtesy of Rob
Grainger, Lyle Zimmerman and Lance Davidson.
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| Lecturers for the 2012 course included:
Heithem
El-Hodiri, Ohio State University
Michael Gilchrist, National Institute for Medical
Research, UK
Robert Grainger, University of Virginia
Raymond Keller, University of Virginia
Mustafa Khokha, Yale University School of Medicine
Peter Klein, University of Pennsylvania
Jonathan Slack, University of Minnesota
John Wallingford, University of Texas at Austin
Lyle Zimmerman, National Institute for Medical Research,
UK
This course is supported with funds provided by the National Institute of Child
Health and Human Development and the Howard Hughes Medical Institute
Cost (including board and lodging): $3,245
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Students
accepted into the course should plan to arrive by early
evening on April 4 and plan to depart after lunch on April
16.
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