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SINGLE CELL ANALYSIS
June 5 - 18, 2013
Application Deadline: March 15, 2013

Instructors:
James Eberwine, University of Pennsylvania
Cynthia McMurray, University of California Berkeley/LBNL

Co-Instructors:
James Hicks, Cold Spring Harbor Laboratory
Jonathan Sweedler, University of Illinois
Stanislav Rubakhin, University of Illinois
Jai-Yoon Sul, University of Pennsylvania
and others tba


Additional Faculty:
tba

Topics:
Transcriptomics
Genomic DNA Sequencing
Epigenomics
Metabolomics
Peptidomics
Protein-Protein Interactions
Functional Genomics
Longitudinal and Multiplex Imaging
Measuring the Metabolic State of Cells In Tissues


The goal of this new two week course is to familiarize students with the most recent cutting edge technologies for characterization of cells. Important in this process will be highlighting the advantages to analysis of single cells in isolation and intheir natural microenvironment. Sections of the course will be taught by scientists who are expert in particular areas of single cell analysis. Topics to be covered include quantitative single cell analysis by RNAseq, genomic DNA analysis, epigenetics, RNA localization analysis, proteomics, protein translation and metabolomics. The techniques to be taught will include real-time live cell quantification where appropriate. Also multiple techniques to isolate specific cell populations and individual cells will be taught.

The course will include the use of model systems such as mouse, Drosophila, C.elegans, C.elegans, Aplysia and Planaria.

This course may be supported with funds provided by:

Cost (including board and lodging): $3,610
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