SINGLE
CELL ANALYSIS
June 5 - 18, 2013
Application Deadline: March 15, 2013
Instructors:
James
Eberwine, University of Pennsylvania
Cynthia
McMurray, University of California Berkeley/LBNL
Co-Instructors:
James Hicks, Cold Spring Harbor Laboratory
Jonathan Sweedler, University of Illinois
Stanislav Rubakhin, University of Illinois
Jai-Yoon Sul, University of Pennsylvania
and others tba
Additional Faculty:
tba
Topics:
Transcriptomics
Genomic DNA Sequencing
Epigenomics
Metabolomics
Peptidomics
Protein-Protein Interactions
Functional Genomics
Longitudinal and Multiplex Imaging
Measuring the Metabolic State of Cells In Tissues
The
goal of this new two week course is to familiarize students
with the most recent cutting edge technologies for characterization
of cells. Important in this process will be highlighting
the advantages to analysis of single cells in isolation
and intheir natural microenvironment. Sections of the course
will be taught by scientists who are expert in particular
areas of single cell analysis. Topics to be covered include
quantitative single cell analysis by RNAseq, genomic DNA
analysis, epigenetics, RNA localization analysis, proteomics,
protein translation and metabolomics. The techniques to
be taught will include real-time live cell quantification
where appropriate. Also multiple techniques to isolate specific
cell populations and individual cells will be taught.
The
course will include the use of model systems such as mouse,
Drosophila, C.elegans, C.elegans,
Aplysia and Planaria.
This
course may be supported with funds provided by:
Cost
(including board and lodging): $3,610
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