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PROTEOMICS
July 9 - 24, 2013
Application Deadline: March 15, 2013

Instructors:
Michael Bereman, University of Washington
Michelle Cilia, Cornell University
Ileana Cristea, Princeton University
Darryl Pappin, Cold Spring Harbor Laboratory

This intensive laboratory and lecture course will focus on cutting-edge proteomic approaches and technologies. Students will gain practical experience purifying and identifying protein complexes and posttranslational modifications. In a section focused on quantitative whole proteome analyses or top-down proteomics, students will gain hands-on experience using two-dimensional gel electrophoresis and mass spectrometry analysis. Students will use differential in-gel electrophoresis (DIGE) for gel-based protein quantification. Differentially expressed proteins will be recognized by statistical methods using advanced gel analysis software and identified using MALDI mass spectrometry. For shotgun proteomic analysis sections or bottom-up proteomics, students will use label-free and covalent isotopic-labeling quantitative approaches to differentially profile changes in protein complexes and whole proteomes. Students will be trained in high-sensitivity microcapillary liquid chromatography coupled with nanospray-ESI and tandem mass spectrometry analysis. Students will learn both single-dimension and multidimensional separation methods. In a section focused on targeted proteomics, students will learn to analyze and process shotgun proteomic data for the development of SRM/MRM assays that accurately identify and quantify targeted proteins. Students will design transitions for selected peptides and perform SRM/MRM mass spectrometry assays. They will learn to process and interpret the acquired data to measure changing quantities of targeted proteins in a variety of biological samples. For all sections of the course, a strong emphasis will be placed on data analysis. A series of outside lecturers will discuss various proteomics topics including: imaging by mass spectrometry, de novo sequence analysis, advanced mass spectrometry methods, protein arrays, and functional proteomics. The aim of the course is to provide each student with the fundamental knowledge and hands-on experience necessary for performing and analyzing proteomic experiments. The overall goal is to train students to identify new opportunities and applications for proteomic approaches in their biological research.

Last year students used and received hands-on training on Agilent QTOF and Thermo Orbitrap, Q-exactive and Vantage triple quadrupole mass spectrometers.

Speakers from 2012 included:
Brian Chait, Rockefeller University
Karl Clauser, Broad Institute, MIT & Harvard
Frank Conlon, University of North Carolina, Chapel Hill
Catherine Costello, Boston University
Craig Dufresne, Thermo Fisher Scientific
Jimmy Eng, University of Washington Proteomics Resource Michael MacCoss, University of Washington
Katalin Medzihradszky, University of California, San Francisco David Muddiman, North Carolina State University
Alexey Nesvizhskii, University of Michigan
Alisha Roberts, Thermo Fisher Scientific
Alejandro Wolf-Yadlin, University of Washington
Joe Zaia, Boston University School of Medicine

This course is supported with funds provided by the National Institute of Child Health & Human Development

Cost (including board and lodging): $3,945
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