How To Apply

Selection Process and Stipends

Travel

General Information

Campus Information

X-RAY METHODS IN STRUCTURAL BIOLOGY
October 14 - 29, 2013
Application Deadline: June 15, 2013

Instructors:
William Furey, V.A. Medical Center, Pittsburgh
Gary Gilliland, Janssen Research & Development, LLC
Alexander McPherson, University of California, Irvine
James Pflugrath, Rigaku Americas Corporation, Texas

See the Roll of Honor - who's taken the course in the past

Crystallography and X-ray diffraction yield a wealth of structural information unobtainable through other methods. This intensified laboratory/computational course focuses on the major techniques used to determine the three-dimensional structures of macromolecules. It is designed for scientists with a working knowledge of protein structure and function, but who are new to macromolecular crystallography. Topics to be covered include basic diffraction theory, crystallization (proteins, nucleic acids and complexes), crystal characterization, X-ray sources and optics, synchrotrons, crystal freezing, data collection, data reduction, multiple isomorphous replacement, multiwavelength anomalous diffraction, molecular replacement, solvent flattening, non-crystallographic symmetry averaging, electron density interpretation, molecular graphics, structure refinement, structure validation, coordinate deposition and structure presentation. Participants learn through extensive hands-on experiments. One or more proteins are crystallized and the structure(s) determined by several methods, in parallel with lectures on the theory and informal discussions behind the techniques. Applicants should be familiar with the creation and editing of simple text files on Linux workstations using a screen based editor (either vi or emacs).

Speakers in the 2012 course included:
Paul Adams, Martin Caffrey, Yu-hang Chen, Aina Cohen, Paul Emsley, Hiro Furukawa, Jeffrey Headd, Wayne Hendrickson, James Holton, Gerard Kleywegt, William Pearson, Randy Read, David Richardson, Robert Sweet, Thomas Terwilliger, Dale Tronrud, David Waugh, John Westbrook

This course is supported with funds provided by the National Institute of General Medical Sciences

Cost (including board and lodging): $3,945
Currency converter

 



Cold Spring Harbor Laboratory
Meetings & Courses Program