July 2 - 18, 2013
Application Deadline: March 15, 2013
Lai, University of Indiana
Lansford, Caltech/University of Southern California
Loturco, University of Connecticut
Stevens, Harvard University
This laboratory and lecture course is designed to provide neuroscientists at all levels with a conceptual and practical understanding of several of the most advanced techniques in molecular neuroscience. The course curriculum is divided into three sections: an extensive and up-to-date set of laboratory exercises, daily lectures covering the theoretical and practical aspects of the various methods used in the laboratory, and a series of evening research seminars. The informal and interactive evening lectures will be given by leading molecular neuroscientists and will serve to illustrate the ways in which the various experimental approaches have been used to advance specific areas of neurobiology. In this year's course, the laboratory portion will include topics such as: an introduction to the design and use of animal virus vectors in neurobiology; the use of small interfering RNAs (siRNA) for regulating the expression of specific genes in neurons; practical exercises in gene delivery systems including mammalian cell transfection protocols and single cell electroporation techniques for targeted gene transfer in vivo; an introduction to overall strategies, use and design of BAC transgenic vectors; quantitative real time RT-PCR analyses from small numbers of cells (RNA purification, PCR optimization, interpretation of results); and application of RNA amplification (aRNA) for single cell analysis. Each laboratory module will be followed by presentations and discussions focused on data interpretation, protocol troubleshooting, and ways to modify and potentially improve existing techniques.
Confirmed Speakers in 2013 include:
James Eberwine, Robert Darnell, Joseph Dougherty, Josh Huang, Sheena Josselyn, Feng Zhang and Kurt Haas
course may be supported with major funding provided by the
National Institute of Mental Health and additional funding
from the Howard Hughes Medical Institute.
and reagents generously provided by New England Biolabs Inc.;
Olympus America Inc.; Carl Zeiss Inc. along with Bitplane-Andor
Technology; Bio-Rad Laboratories; Chroma Technology Corporation;
Coherent Laser; Eppendorf North America; Fotodyne Inc.; Harvard
Apparatus Inc.; Illumina Inc.; Invitrogen Corporation; Leica
Microsystems Inc.; Nanodrop Technologies; Narishige International
USA Inc.; PerkinElmer Life; Promega Corporation; Qiagen Inc.;
Roche Applied Science; Sigma-Aldrich; Thermo Fisher Scientific.
Scientists from developing countries
accepted into this course may be eligible for scholarships
provided by the International Brain Research Organization
(including board and lodging): $3,945