MAST version 3.0 (Release date: 2001/03/05 14:24:28)

For further information on how to interpret these results or to get a copy of the MAST software please access http://meme.sdsc.edu.

If you use this program in your research, please cite:

Timothy L. Bailey and Michael Gribskov, "Combining evidence using p-values: application to sequence homology searches", Bioinformatics, 14(48-54), 1998.

	DATABASE pasted sequences (peptide)
	Last updated on Wed Dec 31 16:00:00 1969
	Database contains 10 sequences, 4033 residues

	MOTIFS  (peptide)
	MOTIF WIDTH BEST POSSIBLE MATCH
	----- ----- -------------------
	  1    50   YDRPSNTTTEIWTLPQVLGERYSGEKDMVVLTSHHTVGVAEDIAYILKKM
	  2    50   RKLRIGQSDFFITVPVSRSLSPLGGGSQTWEGSGVLPCVGIPAEQALEKA
	  3    50   FLVNDIWKKLMGTSSLVLDLQHCTGGEVSGIPFVISYLHQGDILLHVDTV

	PAIRWISE MOTIF CORRELATIONS:
	MOTIF     1     2
	----- ----- -----
	   2   0.19
	   3   0.25  0.20
	No overly similar pairs (correlation > 0.60) found.

	Random model letter frequencies (from non-redundant database):
	A 0.073 C 0.018 D 0.052 E 0.062 F 0.040 G 0.069 H 0.022 I 0.056 K 0.058 
	L 0.092 M 0.023 N 0.046 P 0.051 Q 0.041 R 0.052 S 0.074 T 0.059 V 0.064 
	W 0.013 Y 0.033 

• Each of the following 10 sequences has E-value less than 10.
• The E-value of a sequence is the expected number of sequences in a random database of the same size that would match the motifs as well as the sequence does and is equal to the combined p-value of the sequence times the number of sequences in the database.
• The combined p-value of a sequence measures the strength of the match of the sequence to all the motifs and is calculated by
  • finding the score of the single best match of each motif to the sequence (best matches may overlap),
  • calculating the sequence p-value of each score,
  • forming the product of the p-values,
  • taking the p-value of the product.
• The sequence p-value of a score is defined as the probability of a random sequence of the same length containing some match with as good or better a score.
• The score for the match of a position in a sequence to a motif is computed by by summing the appropriate entry from each column of the position-dependent scoring matrix that represents the motif.
• Sequences shorter than one or more of the motifs are skipped.
• The table is sorted by increasing E-value.

• The ordering and spacing of all non-overlapping motif occurrences are shown for each high-scoring sequence listed in Section I.
• A motif occurrence is defined as a position in the sequence whose match to the motif has POSITION p-value less than 0.0001.
• The POSITION p-value of a match is the probability of a single random subsequence of the length of the motif scoring at least as well as the observed match.
• For each sequence, all motif occurrences are shown unless there are overlaps. In that case, a motif occurrence is shown only if its p-value is less than the product of the p-values of the other (lower-numbered) motif occurrences that it overlaps.
• The table also shows the E-value of each sequence.
• Spacers and motif occurences are indicated by

  • n

    occurrence of motif `n' with p-value less than 0.0001.

  • 1

    Sequences longer than 1000 are not shown to scale and are indicated by thicker lines.

• The positions and p-values of the non-overlapping motif occurrences are shown above the actual sequence for each of the high-scoring sequences from Section I.
• A motif occurrence is defined as a position in the sequence whose match to the motif has POSITION p-value less than 0.0001 as defined in Section II.
• For each sequence, the first line specifies the name of the sequence.
• The second (and possibly more) lines give a description of the sequence.
• Following the description line(s) is a line giving the length, combined p-value, and E-value of the sequence as defined in Section I.
• The next line reproduces the motif diagram from Section II.
• The entire sequence is printed on the following lines.
• Motif occurrences are indicated directly above their positions in the sequence on lines showing
  • the motif number of the occurrence,
  • the position p-value of the occurrence,
  • the best possible match to the motif, and
  • columns whose match to the motif has a positive score (indicated by a plus sign).

                            [3]                                               [1
                            1.6e-60                                           1.
                            FLVNDIWKKLMGTSSLVLDLQHCTGGEVSGIPFVISYLHQGDILLHVDTVYD
                            ++++++++++++++++++++++++++++++++++++++++++++++++++++
76   DGNVGYLRVDYIPGQEVVEKVGEFLVNAIWKNLMGTSSLVLDLQHSTGGEVSGIPFVISYLHQGDILLHVDTVYD

     ]                                                                [2]
     3e-62                                                            3.4e-62
     RPSNTTTEIWTLPQVLGERYSGEKDMVVLTSHHTVGVAEDIAYILKKM                 RKLRIGQSDF
     ++++++++++++++++++++++++++++++++++++++++++++++++                 ++++++++++
151  RPSNTTTEIWTLPQVLGERYGGEKDMVVLTSHRTVGVAEDIAYILKKMRRAIVVGEQTLGGALDLRKLRIGQSDF

     
     
     FITVPVSRSLSPLGGGSQTWEGSGVLPCVGIPAEQALEKA
     ++++++++++++++++++++++++++++++++++++++++
226  FITVPVSRSLSPLGGGSQTWEGSGVLPCVGIPAEQALEKALAILTLRRARPGAIQRLMEVLQNYYTLVDRVPALL

                                      [3]
                                      2.5e-61
                                      FLVNDIWKKLMGTSSLVLDLQHCTGGEVSGIPFVISYLHQGD
                                      ++++++++++++++++++++++++++++++++++++++++++
76   FQQMIKYQVLDGNVGYLRVDYIPGQEVVEKVGEFLVNNIWKNLMGTSSLVLDLQHSTGGEVSGIPFVISYLHQGD

             [1]
             8.1e-61
     ILLHVDTVYDRPSNTTTEIWTLPQVLGERYSGEKDMVVLTSHHTVGVAEDIAYILKKM
     ++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
151  ILLHVDTVYDRPSNTTTEIWTLPQVLGERYGGEKDMVVLTSRRTVGVAEDIAYILKKMRRAIVVGEQTLGGALDL

     [2]
     1.4e-61
     RKLRIGQSDFFITVPVSRSLSPLGGGSQTWEGSGVLPCVGIPAEQALEKA
     ++++++++++++++++++++++++++++++++++++++++++++++++++
226  RKLRIGQSDFFITVPVSRSLSPLGGGSQTWEGSGVLPCVGTPAEQALEKALAILTLRRARPGAIQRLMEVLQNYY

                            [3]                                               [1
                            9.1e-63                                           1.
                            FLVNDIWKKLMGTSSLVLDLQHCTGGEVSGIPFVISYLHQGDILLHVDTVYD
                            ++++++++++++++++++++++++++++++++++++++++++++++++++++
76   EGNVGYLRVDYIPGQEVVEKVGEFLVNDIWKKLMGTSSLVLDLQHSTGGEVSGIPFVISYLHQGDILLHVDTVYD

     ]                                                                [2]
     9e-63                                                            8.3e-56
     RPSNTTTEIWTLPQVLGERYSGEKDMVVLTSHHTVGVAEDIAYILKKM                 RKLRIGQSDF
     ++++++++++++++++++++++++++++++++++++++++++++++++                 +++++ ++++
151  RPSNTTTEIWTLPQVLGERYGGEKDMVVLTSHHTIGVAEDIAYILKKMRRAIVVGEQTLGGALDLRKLRISQSDF

     
     
     FITVPVSRSLSPLGGGSQTWEGSGVLPCVGIPAEQALEKA
     +++++++++++++++++++++++++++++++ +++++ ++
226  FITVPVSRSLSPLGGGSQTWEGSGVLPCVGIAAEQALGKALAILTLRRARPGAIQRLMEVLQDYYTLVDRVPALI

                            [3]                                               [1
                            5.9e-57                                           2.
                            FLVNDIWKKLMGTSSLVLDLQHCTGGEVSGIPFVISYLHQGDILLHVDTVYD
                             +++++++++++++++++++++++++++++++++++++++++++++++++++
76   EGNVGYLRVDYIPGQEVIEKVGELLVNAVWKKLMGTSSLVLDLQHSTGGEVSGIPFVISYLHQGDILLHVDTVYD

     ]                                                                [2]
     8e-62                                                            3.4e-62
     RPSNTTTEIWTLPQVLGERYSGEKDMVVLTSHHTVGVAEDIAYILKKM                 RKLRIGQSDF
     ++++++++++++ +++++++++++++++++++++++++++++++++++                 ++++++++++
151  RPSNTTTEIWTLAQVLGERYGGEKDMVVLTSHHTVGVAEDIAYILKKMRRAIVVGEQTLGGALDLRKLRIGQSDF

     
     
     FITVPVSRSLSPLGGGSQTWEGSGVLPCVGIPAEQALEKA
     ++++++++++++++++++++++++++++++++++++++++
226  FITVPVSRSLSPLGGGSQTWEGSGVLPCVGIPAEQALEKALAILTLRRARPGAIQRLMEVLQNYYTLVDRVPALL

                                                             [3]
                                                             2.9e-56
                                                             FLVNDIWKKLMGTSSLVLD
                                                             +++++++++ + +++++++
1    STSKAPQHDPKFANATQEELLALFQQIIKYQVLEGNVGYLRVDYIPGRDMIEEVGEFLVNDIWKKVMDTSSLVLD

                                    [1]
                                    2.8e-63
     LQHCTGGEVSGIPFVISYLHQGDILLHVDTVYDRPSNTTTEIWTLPQVLGERYSGEKDMVVLTSHHTVGVAEDIA
     +++++ +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
76   LQHSSRGEVSGIPFVISYLHQGDILLHVDTIYDRPSNTTTEIWTLPQVLGERYSGEKDIVVLTSHHTVGVAEDIA

                            [2]
                            2.9e-60
     YILKKM                 RKLRIGQSDFFITVPVSRSLSPLGGGSQTWEGSGVLPCVGIPAEQALEKA
     ++++++                 +++++++++++++++++++++++++++++++++++ ++++++++++++++
151  YILKKMRRAIVVGEQTQGGALDLRKLRIGQSDFFITVPVARSLSPLGGGSQTWEGSGVVPCVGIPAEQALEKALA

                                      [3]
                                      2.6e-58
                                      FLVNDIWKKLMGTSSLVLDLQHCTGGEVSGIPFVISYLHQGD
                                      ++++++++++++++++++++++++ ++++++++++++++ ++
76   LQQMIKYQVLDGNVGYLRVDYIPGQEVVEKVGEFLVNDIWKKLMGTSSLVLDLQHSTEGEVSGIPFVISYLHEGD

             [1]
             2.5e-60
     ILLHVDTVYDRPSNTTTEIWTLPQVLGERYSGEKDMVVLTSHHTVGVAEDIAYILKKM
     +++++++++++++++++++++++++++++++++++ ++++++++++++++++++++++
151  ILLHVDTVYDRPSNTTTEIWTLPQVLGERYSGEKDLVILTSHRTVGVAEDIAYILKKMRRAIVVGEQTLGGALDL

     [2]
     5.6e-61
     RKLRIGQSDFFITVPVSRSLSPLGGGSQTWEGSGVLPCVGIPAEQALEKA
     ++++++++++++++++++++++++++++++++++++++++++++++++++
226  RKLRIGQSDFFITVPVSRSLSPLGGGSHTWEGSGVLPCVGIPAEQALEKALAILTLRRARPAAIQRLMEVLQNNY

                                                             [3]
                                                             1.6e-53
                                                             FLVNDIWKKLMGTSSLVLD
                                                             +++++++++++ +++ +++
1    STSKAPQRDPKLANATQEELLASLQQIIKYQVLEGNVGYLRVDYIPGQEMIEQVGEFLVNDIWKKLMETSSPVLD

                                    [1]
                                    2.8e-63
     LQHCTGGEVSGIPFVISYLHQGDILLHVDTVYDRPSNTTTEIWTLPQVLGERYSGEKDMVVLTSHHTVGVAEDIA
      +++ ++++++++++++++++++ +++++++++++++++++++++++++++++++++++++++++++++++++++
76   PQHSRGGEVSGIPFVISYLHHGDLLLHVDTIYDRPSNTTTEIWTLPQVLGERYSGEKDIVVLTSHHTVGVAEDIA

                            [2]
                            8.0e-62
     YILKKM                 RKLRIGQSDFFITVPVSRSLSPLGGGSQTWEGSGVLPCVGIPAEQALEKA
     ++++++                 ++++++++++++++++++++++++++++++++++++++++++++++++++
151  YILKKMRRAIVVGEQTLGGALDLRKLRIGQSDFFITVPVARSLSPLGGGSQTWEGSGVLPCVGIPAEQALEKALA

                            [3]                                               [1
                            6.7e-57                                           2.
                            FLVNDIWKKLMGTSSLVLDLQHCTGGEVSGIPFVISYLHQGDILLHVDTVYD
                            +++++++++++ ++++++++++++++++++++++++++++ +++++++++++
76   EGNVGYLRVDYIPGQEVIERAGKFLVNDIWKKLMATSSLVLDLQHSMGGQVSGIPFVISYLHQRDILLHVDTVYD

     ]                                                                [2]
     6e-59                                                            1.9e-58
     RPSNTTTEIWTLPQVLGERYSGEKDMVVLTSHHTVGVAEDIAYILKKM                 RKLRIGQSDF
     +++++++++++++++++++++ ++++++++++++++++++++++++++                 ++++++++++
151  RPSNTTTEIWTLPQVLGERYSEEKDMVILTSRHTIGVAEDIAYILKKMRRAIVVGEQTLGGALDLQKLRIGQSDF

     
     
     FITVPVSRSLSPLGGGSQTWEGSGVLPCVGIPAEQALEKA
     ++++++++++++++++++++++++++++++++++++++++
226  FITVPVACSLSPLGGGSQTWEGSGVLPCIGVPAEQALEKALAILTLRKARAGAIQHLMEVLQNYYTLVDRVPALL

                                [3]
                                5.0e-45
                                FLVNDIWKKLMGTSSLVLDLQHCTGGEVSGIPFVISYLHQGDILLHVD
                                +++ +++++++++++++++++++++++++ +++ +++++ + ++++++
76   YKVLEGNVGYLRVDNIPGQEVLNQLGGFLVTHVWKQLMGSSALVLDLRHCTGGHVSSIPYLISYLHPGGTVLHVD

       [1]                                                                [2]
       2.0e-51                                                            3.2e-5
     TVYDRPSNTTTEIWTLPQVLGERYSGEKDMVVLTSHHTVGVAEDIAYILKKM                 RKLRIG
     ++++++++++++ ++++++++++++ +++++++++ ++ ++++++ ++++++                 ++++++
151  TIYNRPSNTTTELWTLPQVLGERYSADKDVVVLTSGHTRGVAEDIVYILKQMGRAIVVGERTEGGALDLQKLRIG

     
     4
     QSDFFITVPVSRSLSPLGGGSQTWEGSGVLPCVGIPAEQALEKA
     +++++++ ++++++++++++++++++++++++++++++ +++++
226  HSDFFLTLPVSRSLGPLGGGSQTWEGSGVLPCVGTPAEEALEKALAILTLRRALPQIIRRLQETLQDYYTLVSRV

                                   [3]
                                   2.1e-39
                                   FLVNDIWKKLMGTSSLVLDLQHCTGGEVSGIPFVISYLHQGDILL
                                   +++  ++ ++ ++++++++ + +++++++++++++++++  ++++
76   SIRHDVLEGNVGYLRVDTIPSQEVVSRLGGFLVASVWRELRGTSALVLDXRXCSGGHVSGIPYVISYLHPENTVL

          [1]                                                                [2]
          3.9e-53                                                            4.5
     HVDTVYDRPSNTTTEIWTLPQVLGERYSGEKDMVVLTSHHTVGVAEDIAYILKKM                 RKL
     ++++++++++++++++++++++ +++++ +++++++++ ++ +++++++++++++                 +++
151  HVDTVYDRPSNTTTEIWTLPQVPGERYSADKDVVVLTSAHTGGVAEDIAHILKQMRRAIVVGEQTVGGALELQKL

     
     e-54
     RIGQSDFFITVPVSRSLSPLGGGSQTWEGSGVLPCVGIPAEQALEKA
     +++++++++++++++++++++ +++ ++++++ ++++++++++++++
226  RIGQSDFFLTVPVSRSLGPLGRGSQVWEGSGVPPCVGTPAEQALEKALAILTLRRALPGVVRRLQEALQDYYARV

CPU: tf036i
Time 0.550000 secs.

mast meme.23244.results meme.23244.data -mf  -df pasted sequences -stdout