SINGLE CELL ANALYSIS
June 6 - 19, 2012
Application Deadline: March 15, 2012

Instructors:
James Eberwine, University of Pennsylvania
Cynthia McMurray, University of California Berkeley/LBNL

Co-Instructors:
Jonathan Sweedler, University of Illinois
Jai-Yoon Sul, University of Pennsylvania

Additional Faculty:
Joerg Bewersdorf, Yale University
Scott Fraser, Caltech
Enrico Gratton, University of California Irvine
James Hicks, Cold Spring Harbor Laboratory
Hari Shroff, NIBIB/NIH
Jan Vijg, Albert Einstein College of Medicine

Topics:
Transcriptomics
Genomic DNA Sequencing
Epigenomics
Metabolomics
Peptidomics
Protein-Protein Interactions
Functional Genomics
Longitudinal and Multiplex Imaging
Measuring the Metabolic State of Cells In Tissues

The goal of this new two week course is to familiarize students with the most recent cutting edge technologies for characterization of cells. Important in this process will be highlighting the advantages to analysis of single cells in isolation and intheir natural microenvironment. Sections of the course will be taught by scientists who are expert in particular areas of single cell analysis. Topics to be covered include quantitative single cell analysis by RNAseq, genomic DNA analysis, epigenetics, RNA localization analysis, proteomics, protein translation and metabolomics. The techniques to be taught will include real-time live cell quantification where appropriate. Also multiple techniques to isolate specific cell populations and individual cells will be taught.

The course will include the use of model systems such as C.elegans, Drosophila and mouse.

This course may be supported with funds provided by:

Cost (including board and lodging): $3,535
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