ADVANCED BACTERIAL GENETICS
June 6 - 26, 2012
Application Deadline: March 15, 2012
Instructors:
Diarmaid Hughes, Uppsala
University, Sweden
Beth
Lazazzera, University of California, Los
Angeles
Fitnat Yildiz,
University of California, Santa Cruz
DO YOU NEED (MORE) TRAINING IN BACTERIAL GENETICS?
The
Advanced Bacterial Genetics course presents logic and
methods used in the genetic dissection of complex biological
processes in diverse bacteria. Laboratory methods include:
classical mutagenesis using transposons, mutator strains,
and chemical mutagens; recombineering with single and
double stranded DNA; detection of gene expression changes
using various reporter genes; the mapping of mutations
using genetic and physical techniques; modern approaches
to the generation and analysis of targeted gene disruptions
and fusions using PCR and cloning methods; epitope insertion
mutagenesis; site-directed mutagenesis; and fluorescence
microscopy. Key components of the course will be the use
of sophisticated genetic methods in the analysis of model
bacteria (including Escherichia coli, Salmonella, Bacillus
subtilis, and Vibrio cholerae), and the use of the wealth
of new genomic sequence information to motivate these
methods.
Invited
lecturers will present various genetic approaches to study
bacterial mechanisms of metabolism, development, and pathogenesis.
Speakers
in the 2011 course included:
Victor DiRita, University
of Michigan Medical School
Michael Ibba, Ohio State University
Petra Levin, Washington University
Stanley Maloy, San Diego State University
Ivan Matic, INSERM, France
George O'Toole, Dartmouth Medical School
Jim Sawitzke, NCI-Frederick
Karen Visick, Loyola University Medical Center
Malcolm Winkler, Indiana University Bloomington
The
course admits 16 students, both foreign and US residents, from diverse
backgrounds and career levels for intensive (but fun) instruction in
microbial genetics.
For
further academic information about the course, feel free to contact the
instructors via their home pages above.
This
course is supported with funds provided by the National Science Foundation.
Cost (including board and
lodging): $4,450
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